高分子量角蛋白在前列腺增生的基底细胞中的表达

Immunohistochemical Analysis of Prostatic Basal Cell

目的 为了探讨前列腺基底细胞的免疫组化特征。方法 采用常规HE染色及免疫组化EnVision两步法 ,用高分子角蛋白 34βE12、广谱CK、Vim、PSA、PsAP、S 10 0、SMA 7种标记物对 37例良性前列腺增生(BTH)的基底细胞进行检测观察。结果 所有 37例BPH的基底细胞对高分子角蛋白 34βE12均呈胞质阳性反应 ,阳性率 10 0 % ;大部分基底细胞 (71% )对广谱CK表达阳性 ;PsAP及PSA在基底细胞中的阳性率均为 35 % ;S 10 0蛋白阳性率较低 ,仅 13.5 % ;而Vim及SMA则均呈阴性反应。结论 高分子角蛋白 34βE12是前列腺基底细胞的特异性标记物 ;前列腺基底细胞具有向前列腺腺泡上皮分化的能力 ,也可引起局部自身增殖 ,故其在前列腺的生长、增生甚至疾病的发生中起着一定的作用 ;前列腺基底细胞与肌上皮细胞是不同的 。

Purpose: To study immunohistochemical quality of prostatic basal cell(PBC). Methods: Therty-seven of basal cell of benign prostatic hypertrophy (BPH) were studied using routine HE stavning and En Vision immunohistochemical methods. Results: The positive rate of 34βE12 in PBC were 100 %; but all of the PBC were negative with Vim and SMA; the positive expression of PsAP PSA in PBC were 35 %; expression of ck in the PBC was 71 % and the positive rate of S-100 in PBC was only 13. 5 %. Conclusions: The resuts suggest that high molecular weight keratin 34βE12 is a specific marker to PBC; PBC has ability to adenoid epithelia diffe-rentiation of prostate and hyperplasia itself. PBC play an important role in growth, hyperplasia and disease of prostate. The PBC unlike myoepithelia, the ability to myoepithelia differentiation is to go a step further controversial opinion.