甲醛灌胃染毒对小鼠骨髓细胞微核率的影响

Effects of intragastric administration of formaldehyde on bone marrow cell micronucleus rate in mice

目的:研究甲醛灌胃染毒对小鼠骨髓细胞微核率的影响。方法:ICR小鼠30只,雌雄各半,随机分为4个组,即空白对照组、阳性对照组、低剂量组和高剂量组,每组10只,雌雄各半,用灌胃的方法给予实验组小鼠甲醛,1次/d,连续5d,阳性对照组给予环磷酰胺。染毒结束后,处死小鼠,取小鼠骨髓进行涂片,在显微镜下观察并计数含微核的嗜多染红细胞,并计算微核率。结果:高剂量组的微核率为(8.41±2.45)‰,低剂量组的微核率为(1.82±1.30)‰,空白对照组的微核率为(0.50±0.85)‰,与空白对照组相比,高剂量组的微核率明显增加,差异具有统计学意义(P<0.05)。结论:甲醛灌胃染毒对小鼠骨髓细胞微核率有一定的影响,可使小鼠骨髓细胞微核率上升。

Objective： To study the effects of intragastric administration of formaldehyde on bone marrow cell micronucleus rate in mice. Methods： Thirty ICR mice （half were male and half were female mice） were randomly divided into 4 groups, one sham group, positive control group, low dose experiment group and high dose two experiment group with 10 mice in each group. The mice were poisoned through intragastric administration once a day for 5 consecutive days. After exposure, the mice were killed and bone marrow smears were made, then cells of bone marrow micronucleus were observed and counted under a microscope. Results： The micronucleus rate in high dose group, low dose group, sham group were （8.41 ±2.45）‰, （1.82±1.30）‰ and （0.50±0.85）‰, respectively, the micronucleus rate in high dose group were statistically significant higher than that of the control group（P〈0. 05）. Oonclusion： Intragastric ad- ministration of formaldehyde to mice can increase the bone marrow micronucleus rate.