盐酸小檗碱纳米乳的抑藻性能研究

Study on Inhibitory Effects of Berberine Hydrochloride Nanoemulsion on Microcystis aeruginosa

选用处方为表面活性剂吐温-80、助表面活性剂甘油、油相大豆油及水,制备盐酸小檗碱纳米乳,并在对该纳米乳质量评价的基础上研究其对铜绿微囊藻的抑制效应。结果表明:在四相质量比为36∶9∶5∶10情况下制备出的水包油型盐酸小檗碱纳米乳澄清透明,分散均匀,平均粒径约为(14.2±3.8)nm,载药量为3 980 mg/L。该纳米乳能大幅提高盐酸小檗碱的抑藻性能,2 mg/L盐酸小檗碱纳米乳抑藻性能与8 mg/L未纳米化盐酸小檗碱抑藻性能相当,同时结果表明盐酸小檗碱纳米乳对无毒微囊藻的抑制效应强于有毒微囊藻。该研究将纳米药物技术应用于有害藻类的防控并取得较好效果,为纳米药物技术在水产养殖业应用提供科学参考。

With the prescription for preparing berberine hydrochloride nano-emulsion, surfactant tween-80, cosurfactant glycerol, oil phase soybean oil, and water were selected, and then laboratory studies about inhibitory effects of berberine hydrochloride nanoemulsion on Microcystis aeruginosa were carded out for evaluating quality of the nano-emulsion in inhibiting algae growth. The results showed that O/W berberine hydrochloride nano-emulsion prepared was clear, transparent and uniformly dispersed; mean size of the berberine hydrochloride nanoemulsion droplet was （14.2_＋3.8） nm; and the drug loading of the nanoemulsion was 3 980 mg/L. Compared to the common berberine bydrochloride, berberine hydrochloride nanoemulsion had exhibited much higher inhibitory effect on the M. aeruginosa, e.g. the efficacy of 2 mg/L berberine hydrochloride nanoemulsion was equivalent to that of 8 mg/L berberine hydrochloride. Meanwhile, the berberine hydrochloride nano-emulsion exhibited higher inhibitory effect on non-toxic M. aeruginosa strain 469 than on toxic strain 905. Accordingly, nano-medicines technology could be successfully applied to controlling harmful algae, thus providing scientific reference for application of nano-medicines technology in aquaculture.