大肠癌细胞核DNA含量检测的价值

Value of Measuring Nuclear DNA Content in Colorectal Cancer

目的 研究大肠癌与细胞核DNA含量的关系。方法 应用图像细胞仪检测 2 1例正常肠黏膜及 32例大肠癌细胞核DNA含量。结果 大肠癌组DI、PI、S %、>5C %及非整倍体检出率均明显高于正常肠黏膜组 ,大肠癌DNA直方图呈Ⅲ、Ⅳ型分布 ,正常肠黏膜呈Ⅰ型分布。结论 细胞核DNA含量与肿瘤分化程度、病灶大小、病灶部位及预后有关 ,与患者年龄、性别无关。

Purpose: To study the relationship between the nuclear DNA contents and colorectal cancer. Methods: The nuclear DNA contents were measured by automatic image analysis technique in 21 cases with normal colorectal mucosa, and in 32 cases with colorectal cancer. Results: The DNA index (DI), proliferation index (PI), S%, >5C% and detection rate of aneuploidy were significantly higher than those of normal colorectal mucosa group. DNA polidy pattern of colorectal cancer group were type III and IV, but that of normal colorectal mucosa group was only type I. Conclusions: The DNA content was related to the degree of differentiation of tumor, size of tumor, situs of tumor and prognosis, not related to age or sex. Measuring nuclear DNA content in colorectal cancer was useful for diagnosing colorectal cancer, understanding the biological characteristic, assessing patient prognosis.