大骨节病区粮水对猴软骨糖胺多糖代谢的影响

Influence of grains or water from KBD endemic area on glycosaminoglycan metabolism in Rhesus monkey cartilages

目的 探讨糖胺多糖 (GAG)代谢与大骨节病发病机制的关系。方法 选用雄性恒河幼猴 ,饲以大骨节病区粮、水 18个月 ,复制出大骨节病动物模型 ,用高效液相色谱法测定实验动物恒河猴软骨糖胺多糖酶解产物中每种二糖的含量。结果 与对照组相比 ,病区粮组和病区水组的猴股骨头、胫骨平台、肋软骨糖胺多糖分子都出现低硫酸化 ,股骨头和胫骨平台软骨 GAG中主要是不饱和 4位硫酸化二糖 (△ Di- 4S)成分减少 ,肋软骨 GAG中主要是不饱和 6位硫酸化二糖 (△ Di- 6 S)成分减少。结论 病区粮和病区水中的致病因素促使猴软骨 GAG分子低硫酸化 ,软骨

Objective To approach the relationship between glycosaminoglycan metabolism in cartilages and pathogenesis of KBD.Methods Rhesus monkey was fed with grains and water from KBD endemic area for 18 months to produce the animal model with KBD . The glycosaminoglycans in the monkey cartilage were extracted by the improved Dish method of Bitter. Purified glycosaminoglycans were digested with chondroitinaseABC, and the enzymatic digests were analyzed by HPLC. Results Comparing with those of the control, the glycosaminoglycans in the head of femur, tibia plateau and costal cartilage from the Rhesus monkey fed with grains and water from KBD endemic area were undersulfated . Decreased unsaturated 4 sulphated disaccharide (△Di 4S) from the glycosaminoglycans in the head of femur and tibia plateau and decreased unsaturated 6 sulphated disaccharide (△Di 6S) from the glycosaminoglycans in the costal cartilage were discovered.Conclusions Detrimental factors in grains and water from KBD endemic area cause undersulfate of the cartilages glycosaminoglycans from Rhesus monkey. The glycosaminoglycans changes have a direct bearing on the pathological alterations in morphology on the cartilages from the animal model with KBD.