自由基在慢性氟中毒大鼠肾脏损伤中的作用

The role of free radicals in the pathogenesis of kidney injury in chromie fluorosis

目的 用电子自旋共振 (ESR)法直接检测自由基含量 ,研究慢性氟中毒大鼠肾脏损伤与自由基的关系 ,从而确定自由基在肾脏损伤中的作用。方法 Wistar大鼠分为 3组。正常对照组 ,未作任何处理 ;染氟组 ,自由饮用含氟化钠 132 .6 mg/L 的含氟水 ;抗氧化中药治疗—染氟组 ,自由饮用 132 .6 mg/L 的含氟水 ,同时每日经腹腔内注射抗氧化中药丹参绞股蓝复方 3.12 5 m l/kg,实验 6个月时检查动物。结果 与正常对照组相比 ,自由基 ,染氟组明显升高 ,抗氧化中药—染氟组则无明显差异 ;血、肾超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶(GSH- Px)活性 ,染氟组明显降低 ,抗氧化中药—染氟组则无明显变化 ;丙二醛 (MDA) ,染氟组明显升高 ,抗氧化中药—染氟组则无明显改变 ;电镜下见染氟大鼠肾小管上皮细胞内线粒体肿胀 ,嵴变短、溶解、消失 ,有巨大线粒体 ,内质网扩张 ,粗面内质网核糖体脱落等变化。抗氧化中药处理的染氟组未见肾脏病变。结论 氟中毒的肾脏病变可能是自由基损伤的结果。

Objective To study the correlation between the free radicals and kidney injury in chronic fluorosis.Methods 30 Wistar rats were divided into three groups at random.Normal control group,drinking water containing sodium fluoride less than 1 mg/L.Fluoride exposed group,drinking water containing sodium fluoride,60 mg/L.Antioxidant fluoride exposed group,drinking water containing sodium fluoride,60 mg/L,meanwhile daily intra abdominal injection of Danshen Gypenosides compound,3.125 mg/kg.The animals were examined by the sixth month.Results As compared with the normal controls,the fluoride exposed rats showed higher levels of free radicals and MDA,lower activity of SOD and GSH Px in the blood and kidney tissues.The kidneys showed swelling of mitochondria and expansion of endoplasmic reticula.Whereas,the antioxidant herbs treated group showed no abnormal change.Conclusions The fact indicated that kidney injury was closely correlated to free radicals.