茶树愈伤组织诱导培养及儿茶素合成的研究

Tea Callus Inducing Culture and Catechins Synthesis

以茶鲜叶不同部位为外植体,在不同消毒方法下诱导产生愈伤组织。同时研究了不同茶树品种、不同培养基和不同激素组合对儿茶素合成的影响。通过试验优化,以茶树第二叶作为外植体,35%次氯酸钠,浸泡18min,可获得较好的诱导效率。在继代培养中,金牡丹愈伤组织中儿茶素含量最高。除黄玫瑰外,其它五种茶树品种使用B5培养基儿茶素含量均高于MS培养基。以金牡丹茶鲜叶为材料,使用B5培养基,2,4-D浓度为0.5mg/L,KT浓度为1.0mg/L时,茶树愈伤组织中儿茶素含量最高。

Different parts of fresh tea leaves were induced in the different disinfection methods to produce callus. Meanwhile, The effects of different tea varieties, different medium and different hormone combinations on catechins synthesis were investigated. Results showed that the second tea leaves as explants, 35%sodium hypochlorite, soak for 18 minutes, could be obtained better efficiency of the induction. In the subculture, the catechin content of Camellia sinensis var. Jinmudan callus was the highest among tea callus. Except Camellia sinensis var. Huangmeigui, the catechin content in the other five tea varieties using B5 medium was higher than that using the MS medium. We obtained the maximum catechins production,when the B5 medium used for Camellia sinensis var. Jinmudan callus cultivation was supplemented with 2,4-D 0.5 mg/L and KT 1.0mg/L.