Survivin、NF-кB和STAT3 mRNA在宫颈癌中的表达及其临床意义

The expression and significance of Survivin,NF-κB a nd S TAT3 mRNA in cervical cancer

目的：检测Survivin、NF-κB、STAT3 mRNA在40例子宫颈癌组织及30例正常子宫颈组织中的表达，分析它们与患者临床病理指标的相关性，初步探讨它们在子宫颈癌发生发展中的相互关系及作用机制。方法：经Trizol一步法提取40例子宫颈癌组织及30例正常子宫颈组织标本的总RNA，逆转录成cDNA，反转录RT-PCR法检测子宫颈癌组织及正常子宫颈组织中Survivin、NF-κB、STAT3 mRNA表达，分析Survivin、NF-κB、STAT3 mRNA表达及与临床病理指标之间的相关性。结果：子宫颈癌组织中Survivin、NF-κB、STAT3 mRNA表达水平显著高于正常子宫颈组织（P〈0.05）。Ⅱb～Ⅲa期子宫颈癌组织中三者mRNA的表达显著高于Ⅰ～Ⅱa期（P〈0.01）；子宫颈癌患者中有淋巴结转移者Survivin mRNA的表达明显高于无淋巴结转移者（P〈0.01）。且STAT3表达与Survivin表达相关（r=0.483，P〈0.05）；Survivin表达与NF-κB表达相关（r=0.377，P〈0.05）。结论：Survivin、NF-κB、STAT3 mRNA在子宫颈癌组织中表达上调，共同参与子宫颈癌的发生发展。

OBJECTIVE：To test expressions of Survivin,NF-κB and STAT3 in 40 cervical carcinoma tissue and 30 normal cervical tissue samples. The correlation between Survivin, NF-κB and STAT3 expression level and the clinical factors and pathology classification were analyzed,and the relationship and mechanism of Survivin,NF-κB and STAT3 in cervical carcinoma development were studied. METHODS：Trizol one step method was used to extract total RNA from both cervical carcinoma and normal tissues,mRNA reverse transcriptase cDNA,the polymerase chain reaction （RT-PCR half quantitative） method was used to measure Survivin, NF-κB and STAT3 mRNA expressions in cervical carcinoma and normal tissues. Further analysis of correlation between Survivin, NF-κB and STAT3 mRNA expression and the clinical pathological factors were performed. RESULTS：Survivin, NF-κB and STAT3 mRNA expressions in carcinoma tissue were significantly higher than the normal tissue （P〈0.05）. Survivin,NF-κB and STAT3 mRNA expression inⅡb-Ⅲa stage was significantly higher thanⅠ-Ⅱa stage （P〈0.01）. The expression of Survivin mRNA in patients with lymph node metastasis was higher than those with no lymph node metastasis （P〈0.01）. Survivin expression was related to STAT3 expression （r=0.483,P〈0.05） and to NF-κB expression （r=0.377,P〈0.05）. CONCLUSION：Survivin,NF-κB and STAT3 expressions were upregulated in cervical carcinoma. All of these were closely related to the development and progression of cervical carcinoma.