红丝线草对大鼠的致畸性研究

Teratogenicity of peristrophe roxburghiana in rats

目的:检测红丝线草的致畸性。方法:红丝线草提取液设10.0、5.0、2.5 g/kg 3个剂量组,同时以去离子水作阴性对照,阿司匹林(0.3 g/kg)作阳性对照。每组12只SD孕鼠,受孕6 d开始按10 mL/kg给孕鼠灌胃受试溶液,每天1次,至受孕15 d,共灌胃10次。于妊娠20 d解剖孕鼠,检查胎鼠的身体、外观、内脏及骨骼发育等指标。结果:红丝线草各剂量组的孕鼠体质量、窝质量、胎鼠体质量、身长、尾长、活胎率、吸收胎率及死胎率与阴性对照组比较差异均无统计学意义(P均>0.05),未见胎鼠外观、内脏和骨骼发育异常及畸形。结论:在本实验条件下,红丝线草对大鼠无母体毒性、胚胎毒性和致畸性。

OBJECTIVE：To determine the teratogenic effects of peristrophe roxburghiana （Schult.） brem in rats. METHODS：Pregnant rats were divided into 5 groups,each with 12 rats. Three groups were treated orally with th thexract from peristrophe roxburghiana for 10 days from 6 to 15 day of pregnancy with doses of 10.0,5.0,2.5 g/kg. The other two groups were fed orally with deionized water as negative control and aspirin with 0.3 g/kg as positive control. At th20 day,the rats were sacrificed to allow physical examination of the fetuses. RESULTS：In all treated groups the body weight of parental rats, litter weight, live rate, fetus body weight and fetus length were not significantly different from negative control group （P〉0.05）,and no abnormality of organ or external was found in the fetuses. CONCLUSION：In these experimental conditions,peristrophe roxburghiana had no appearance maternal toxicity,embryo toxicity nor teratogenicity in rats.