人博卡病毒2基因组扩增及序列分析

Genome Amplification and Sequence Analysis of Human Bocavirus 2

为获得人博卡病毒2(Human bocavirus 2,HBoV2)基因组序列,以2010年HBoV2单阳粪便标本为材料,PCR方法扩增HBoV2基因组不同区域,经序列拼接后得到5444bp的全基因组序列(HBoV2-NC)。系统进化分析显示,HBoV2-NC与兰州株HBoV2亲缘关系最近;DINAMelt末端回文结构预测证实,HBoV2-NC 5′末端存在反向互补序列,具有典型的细小病毒末端的茎环样结构;接头法PCR扩增得到HBoV2-NC部分末端侧翼序列。

To obtain the genome sequence of human bocavirus 2 （HBoV2）, different regions of HBoV2 genome were amplified through PCR in fecal specimens which had been identified as single-ositive for HBoV2 in 2010. A genome sequence of HEoV2 （HBoV2-NC, 5444 bp） was obtained after sequence assembly. The phylogenetic analysis showed that HBoV2-NC had the closest evolutionary relationship with HBoV2 Lanzhou strain. The predication of inverted terminal repeats of HBoV2-NC hy DINAMeIt showed that inverted terminal repeats were contained in HBoV2-NC 5＇ terminal, which had the typical stem-loop structure in other parvoviruses. Finally, some flanking sequences of HBoV2-NC were amplified by linker-PCR.