白癜风表皮黑素细胞及真皮噬黑素细胞的研究

Investigation of epidermal melanocytes and dermal melanophages in the vitiligo

目的观察白癜风患者表皮黑素细胞及真皮噬黑素细胞改变并探讨其意义。方法白癜风患者38例，分为寻常型进展期组、稳定期组，节段型进展期组及稳定期组。另选取同期整形外科手术的正常皮肤10例为正常对照组。采用免疫组化染色及常规病理HE染色，对Melan—A阳性细胞及真皮噬黑素细胞的数量进行统计分析。结果与正常对照组相比，白癜风各组Melan—A阳性细胞数量明显减少，噬黑素细胞数量明显增多，差异均有统计学意义（P〈0．01）。白癜风各组别之间皮损Melan—A阳性细胞数差异无统计学意义（P〉0．05）。同类型进展期组噬黑素细胞高于稳定期组，差异有统计学意义（P〈0．01）。结论白癜风患者皮损中表皮黑素细胞明显减少，真皮噬黑素细胞明显增多，可能存在黑素细胞的真皮丢失途径。

Objective To observe the changes of epidermal melanocytes and dermal melanoph- ages in vitiligo and investigate its significance. Methods Thirty-eight cases of vitiligo were divided into progressive stage group, stable stage group in nonsegmental vitiligo patients, and progressive stage group, stable stage group in segmental vitiligo patients. Normal controls of 10 cases were selected from the plas- tic surgery deparment in the same period. Melan-A positive cells and dermal melanophages were deter- mined by immunohistochemical staining and HE staining. Results Compared with normal controls, Me- lan-A positive cells counts were apparently lower and melanophages counts were higher in each vitiligo groups than those of normal controls, the differences were significant（ P 〈 0.01 ）. No significant differ- ences were found among vitiligo groups （ P 〉 0. 05 ）. Melanophages counts of each vitiligo groups in pro- gressive stage were significantly higher than those of stable stage, the differences were significant （P 〈 0.01 ）. Conclusions Apparently decreased counts of epidermal melanocytes and increased counts of dermal melanophages are found in vitiligo lesions. The lost of melanocytes from dermal pathway may par- ticipate in the pathogenesis of vitiligo.