小G蛋白Rad基因对心肌肥大的影响

Effect of small G protein Rad gene on cardiac hypertrophy

目的研究Rad基因对心肌细胞肥大的影响,为心肌肥大早期干预提供理论基础。方法针对Rad基因序列构建Rad过表达和低表达腺病毒载体,体外培养乳鼠心肌细胞,分为8组:空白对照组,Ad-GFP组,Ad-RNA干扰(RNAi)组,Ad-Rad组,肥大模型组(心肌营养素1诱导),肥大+Ad-GFP组,肥大+Ad-RNAi组,肥大+Ad-Rad组,48h后检测Rad、心房利钠因子(ANF)mRNA和蛋白表达水平。结果肥大模型组ANF蛋白表达较空白对照组明显升高;肥大+Ad-Rad组ANF蛋白表达较肥大+Ad-GFP组明显降低,肥大+Ad-RNAi组ANF蛋白表达较肥大+Ad-GFP组明显升高(P<0.05)。肥大模型组Rad mRNA和蛋白表达较空白对照组明显降低;Ad-Rad组Rad mRNA和蛋白表达较Ad-GFP组明显增加,Ad-RNAi组Rad mRNA和蛋白表达较Ad-GFP组明显降低(P<0.05)。结论 Rad基因可抑制在细胞水平心肌营养素1诱导的心肌细胞肥大反应。

Objective To provide the theoretical evidence for early treatment of myocardial hypertrophy by studying the effect of Rad gene on myocardial hypertrophy .Methods Adenovirus vectors were constructed for over and low expression of Rad .In vitro cultured suckling rat myocardial cells were divided into blank control group ,Ad-GFP group ,Ad-RNAi group ,Ad-Rad group ,hypertrophy model group （induced by cardiotrophin 1） ,hypertrophy＋ Ad-GFP group ,hypertrophy＋ Ad-RNAi group ,hypertrophy＋ Ad-Rad group .Expressions of Rad and ANF mRNA and protein were detected 48 h after transfection .Results The expression level of ANF protein was significantly higher in hypertrophy model group than in blank control group ,significantly lower in hypertrophy＋Ad-Rad group than in hypertrophy＋Ad-GFP group and significantly higher in hypertrophy＋ Ad-RNAi group than in hypertrophy ＋ Ad-GFP group （P〈 0 .05） .The expression level of Rad mRNA and protein was significantly lower in hypertrophy model group than in blank control group ,significantly higher in hypertrophy＋Ad-Rad group than in hypertrophy＋Ad-GFP group and significantly lower in hypertrophy ＋ Ad-RNAi group than in hypertrophy ＋ Ad-GFP group （P〈0 .05） .Conclusion Rad gene can inhibit cardiotrophin 1-induced myocardial hypertrophy at cellular level .