离子色谱法测定血清锂离子候选参考方法的建立及性能评估

Development and evaluation of candidate reference method for the determination of lithium in serum based on ion chromatography

目的：以离子色谱法为基础，建立血清总锂离子测定的候选参考方法并评价其性能。方法采用一种简单的样本前处理方法，用新鲜患者血清样本、IFCC-RELA 样本等对方法的精密度进行评估；用加标回收实验评价方法的准确性。结果锂离子标准曲线线性方程为：Y ＝0．8171X -0．0013，r^2＝0．99995，方法的检出限为6μg/L；测量血清样本的不精密度（CV）小于1．0％；样本的加标回收率在99％～101％之间。结论成功建立了血清锂候选参考方法，可用于血清锂离子项目的量值溯源和标准化，为血清锂常规检测系统向参考方法/参考物质溯源提供了有效途径。

Objective To establish a candidate reference method for the determination of serum lithium based on ion chromatog-raphy and evaluate its analytical performance.Methods A simple sample treatment procedure,which can be remove the proteins and/or organics in human serum,has been developed for the determination of serum lithium.Method precision was evaluated with different concentration of fresh human serum and EQA sample RELA-A/B.Method accuracy was investigated with the recovery experiments in fresh human serum and RELA-A/B sample.Results The linear equation was Y =0.817 1X -0.001 3 with a correlation coefficient of 0.999 95 under the optimum experimental conditions,the detection limit （3S/N）for lithium was 6 μg/L and the imprecision was less than 1.0%.The results of the recovery experiments indicated that the recoveries were reasonable for the deter-mination of serum lithium,in a range of 99%-101%.Conclusion The candidate reference method of lithium was successfully established and which can be used for traceability and standardization.It may provide an effective way for routine testing of lithium traceable to the reference method/reference material.