免疫荧光分析法在快速诊断A、B型流感病毒中的临床应用

The Clinical Application in Rapid Diagnosis Flu Virus A and Flu Virus B by Fluorescent Immunoassay Analysis

目的探讨Sofia Influenza A+B FIA检测系统在快速诊断A、B型流感病毒中的临床应用价值。方法获取临床168例流感患儿的鼻咽拭子样本,分别通过Sofia Influenza A+B FIA检测系统和胶体金免疫层析(GICA)法分别进行A、B型流感病毒检测,并通过逆转录聚合酶链反应(RT-PCR)进行验证。结果 Sofia Influenza A+B FIA检测系统的阳性检出率为17.86%(30/168),高于GICA法;同时,与RT-PCR相比,对A型流感病毒和B型流感病毒的灵敏度分别达到83.3%和71.4%,特异性均达到100.0%。结论 Sofia Influenza A+B FIA检测系统是一种灵敏度和特异性均较高,能够快速检测A、B型流感病毒的方法。

Objective To evaluate the clinical application value of Sofia Influenza A＋B fIA in detecting flu virus A and flu virus B.Methods Flu virus A and flu virus B was detected in 168 samples of nasopharyneal secretions which collected from children with influenza infection by Sofia Influenza A＋B FIA,GICA and RT-PCR methods.Results Sofia Influenza A＋B FIA positive detection rate was 17.86%（30/168）,higher than GICA method.Compared with RT-PCR,its sensitivity and specificity for flu virus A and flu virus B were 83.3%,100% and 71.4%,100% respectively.Conclusion Sofia Influenza A＋B FIA is a rapid clinical application method with high sensitivity and specificity.