含化上清片中白花前胡甲素含量测定方法的研究

Study on Quantitative Methods of Praeruptorin A in Hanhua Shangqing Tablets by RP-HPLC

目的建立高效液相色谱法测定含化上清片中白花前胡甲素的含量。方法采用反相高效液相色谱法，对样品处理方法、色谱柱和流动相等影响分离效果的主要因素进行了优化。选用Thermo GOLD HPERSIL C18色谱柱（4．6mm×250mm，5μm）；乙腈-0．1％磷酸溶液（57：43）为流动相；流速为1．0mL·min^-1；检测波长为320nm。结果白花前胡甲素在0．0205～0．1640μg范围内呈良好的线性关系（r=0．99997），平均回收率为96．88％，RSD为0．58％（n=8）。结论该方法经方法学验证可用于含化上清片中前胡的质量控制。

Objective To establish an HPLC method for the determination of praeruptorin A in Hanhua Shangqing Tablets. Methods The main factors （including extracting method, chromatographic column and mobile phase） influencing chromatographic separation effect were optimized by RP-HPLC. The determination was performed on the Thermo GOLD HPERSIL C18 column （4.6 mm × 250 mm, 5 μm）. The mobile phase was acetonitrile-0.1% phosphoric acid solution （57 ： 43） with the flowing rate at 1.0 mL · min^-1. The detective wavelength was 320 nm. Results The calibration curve of praeruptorin A was linear ranged in 0.0205-0.1640 μg （r=0.99997）. The average recovery was 96.88%, RSD=0.58% （n= 8）. Conclusion This methodologically validated method can be used for quality control of Hanhua Shangqing Tablets.