辽宁满族人群19个STR基因座的遗传多态性及其与28个群体的遗传关系研究

Genetic Polymorphism of 19 STR Loci in Manchu Nationality in Liaoning and Their Genetic Relationship with Other 28 Populations

目的调查辽宁地区满族无关个体的19个短串联重复序列(STR)基因座多态性,选择具有高度遗传多态性与稳定性的13个STR位点进行辽宁满族与来自28个不同国家和地区的人群之间的群体遗传关系分析,研究其在法医学个体识别、亲子鉴定和人类学研究中的应用价值。方法应用GoldeneyeTM20A五色荧光标记系统对采集的150例口腔黏膜细胞样本进行19个基因座的复合扩增,应用ABI3130XL全自动测序仪对扩增产物进行检测,采用GeneMapper v3.2软件对其进行基因分型。用Neighbor.Joining法构建系统发生树,并结合有关资料分析他们之间的遗传关系。结果GoldeneyeTM20A系统的19个STR基因座在辽宁地区满族人群的累积个体识别能力(TDP)为0.999 999 999 999 999 999 994 2,累积非父排除率(CPE)为0.999 999996 777。结论辽宁满族的19个STR位点具有高度遗传多态性,可用于辽宁地区满族人群法医学个体识别、亲权鉴定及人类学研究。

Objective To investigate the genetic polymorphism of 19 short tandem repeat （STR） loci in Liaoning Manchu population with GoldeneyeTM20A multiplex amplification system, and to determine the molecular genetic relationship of Liaoning Manchu with other 28 populations around the world. Methods DNA samples were obtained from 150 unrelated individuals. PCR products of the 19 loci were analyzed by ABI3130XL sequencer. The genetic distances among 29 different populations were calculated by Nei＇ s method. The phylogenetic tree was constructed using Neighbor-Joining method. Results These loci were highly polymorphic. The combined power of discrimination is 0.999 999 999 999 999 999 994 2 and the combined paternity of exclusion is 0.999 999 996 777. Conclusion The 19 STR loci of Manchu nationality in Liaoning possess the characteristics of high genetic diversity, which can be used for anthropological and other comparative studies of populations, and is also useful for forensic and paternity testing of the Manchu population in China.