摘要
目的研究miR-218在肾透明细胞癌中的表达及其与临床病理之间的关系。方法收集2012年3月—2013年5月间在该院就诊的46例病理确诊为肾透明细胞癌组织和30例癌旁≥3.0 cm正常肾组织作为对照组,采用Realtime PCR方法检测microRNA-218在肾透明细胞癌组织和癌旁≥3.0 cm正常肾组织的表达,分析microRNA-218表达水平与肾透明细胞癌临床病理参数之间的关系。结果与对照组癌旁正常肾组织相比,miR-218在肾透明细胞癌组织的的表达明显降低(P<0.001)。同时,miR-218表达的降低与肿瘤的分期,转移和分化程度有密切关系。miR-218的表达与患者的年龄,性别,肿瘤位置及肿瘤大小无明显统计学意义。但是,miR-218的表达与肿瘤的转移(P=0.007),肿瘤的T分期(P=0.022)及肿瘤的分级(P=0.015)有统计学意义。结论 miR-218在肾透明细胞癌中和肿瘤分期、分化、转移有着很大的相关性。miR-218在肾透明细胞癌组织呈下调表达,其可能成为肾细胞癌新的潜在的治疗靶点及诊断预后的分子标志物。
Objective To investigate the expression of miR-218 in renal clear cell carcinoma and its relationship with clinical pathology. Methods The renal clear cell carcinoma tissue of 46 cases of patients diagnosed with renal clear cell carcinoma in our hospital from March, 2012 to May, 2013 were selected and 30 cases of normal renal tissues ≥3.0cm adjacent to carcinoma as the control group. Realtime PCR method was used to detect the expression of microRNA-218 in renal clear cell carcinoma tissue and normal renal tissue ≥ 3.0cm adjacent to carcinoma. The relationship between the expression of microRNA-218 and renal clear cell carcinoma clinicopathological parameters was analyzed. Results Compared with the control tissues, miR-218 expression in the RCCC tissues was significantly lower （P〈0.001）. Moreover, low miR-218 was closely associated with the stage, metastasis, and the grade of the tumor. The expression of miR-218 with the patient＆#39;s age and gender, tumor location and tumor size had no statistical significance. But the expression of miR-218 with the tumor metastasis （P=0.007）, tumor T staging （P=0.022） and tumor grade （P=0.015）, had statistical significance. Conclusion MiR-218 was strongly correlated to RCCC stage, tumor grade, tumor metastasis. MiR-218 was low express in the tissues of RCCC,it may be used as a potential therapeutic target,a diagnostic biomarker and a prognostic predictor in patients with RCCC in future.
出处
《中外医疗》
2014年第11期20-21,共2页
China & Foreign Medical Treatment
