菁染料五甲川菁的遗传毒性研究

The genetic toxicity of 5-polymethine cyanine

目的:研究菁染料五甲川菁(Cy5)的遗传毒性,为其安全利用提供科学依据。方法:选用小鼠50只,随机分为阴性对照组(生理盐水)、阳性对照组(环磷酰胺,CP)和Cy5不同剂量试验组(2、20、200 mg/kg)。采用小鼠骨髓细胞微核试验、小鼠精子畸形试验、小鼠骨髓染色体畸变试验和Ames试验,检测Cy5的遗传毒性作用。结果:在小鼠骨髓细胞微核试验、小鼠精子畸形试验和小鼠骨髓染色体畸变试验中,Cy5各试验组与阴性对照组相比以及各试验组两两之间比较差异均无统计学意义(P均>0.05),而各试验阳性对照组与阴性对照组相比差异均有统计学意义(P<0.05);Ames试验结果亦呈阴性反应。结论:在本试验条件下,未观察到Cy5对小鼠的遗传毒性作用。

OBJECTIVE： To study the genetic toxicity of 5-polymethine cyanine（Cy5）,and to provide scientific evidence for its safe use. METHODS：Mice were randomly divided into five groups,including negative group （saline）,positive group （cyclophosphamide,CP） and test groups with different doses of Cy5 （2,20,200 mg/kg）. Micronucleus test in mice bone marrow polychromatic erythrocytes,mice sperm shape abnormality test,mice marrow chromosome aberration test and Ames test were used to investigate the genetic toxicity of Cy5. RESULTS：Bone marrow micronucleus test,sperm shape abnormality test and marrow chromosome aberration test,in each Cy5 test group compared with the negative control group and the differences between the test groups were statistically insignificant（P〉0.05）,while each positive group compared with the negative control group was statistically significant （P〈0.05）. Ames test showed negative reaction. CONCLUSION：Under the conditions of this experiment,Cy5 has genetic toxic effect was not found in mice.