摘要
利用阳极氧化法在纯Ti表面制得TiO2纳米管,随后用冻干法在纳米管中负载microRNA制得复合涂层。利用SEM、TEM和荧光显微镜对TiO2/microRNA复合涂层进行表征,并对microRNA的释放行为以及其进入成骨细胞后的功效进行分析。结果表明,microRNA分子成功负载在TiO2纳米管中,并在12h内持续稳定释放投递,荧光照片表明投递的microRNA成功进入成骨细胞,ALP活性测定进一步表明进入细胞中microRNA促进了成骨分化。
TiO2 nanotubes was prepared by anodic oxidation method on pure Ti surface,then the microRNA was loaded in the nanotubes by lyophilization to build composite coating.The TiO2/microRNA composite coating was characterized by SEM,TEM and fluorescence microscope.The release behavior of microRNA and its furthrer function was analysised.The results showed that microRNA successfully deposited in TiO2 nanotubes, and sustained release delivery for 1 2 h.Fluorescent images indicated that the microRNA was transfected into osteoblasts,ALP activity assay further indicated that the microRNA promoted the osteogenic differentiation.
出处
《功能材料》
EI
CAS
CSCD
北大核心
2014年第6期75-78,共4页
Journal of Functional Materials
基金
国家国际科技合作专项资助项目(2012DFA51690)
