摘要
为探讨黄花梨(Pyrus pyrifolia‘Huanghua’)PpbZIP基因的功能,根据同源基因bZIP从黄花梨花芽中克隆到PpbZIP基因(GenBank登录号:KC951877)。PpbZIP基因的全长cDNA为1843 bp,其中开放阅读框长度1227 bp,编码408个氨基酸,与同为蔷薇科的苹果bZIP蛋白序列的同源性高达92%。PpbZIP基因的QRT-PCR分析表明,PpbZIP基因在黄花梨花芽从休眠到休眠解除后的表达量呈先升高后降低的趋势。这表明PpbZIP参与了黄花梨花芽休眠过程的调控。
In order to understand the function of PpbZIP of Pyrus pyrifolia ‘Huanghua’, PpbZIP gene was clonedfrom flower buds of ‘Huanghua’ based on homologous gene of bZIP. The results showed that full length of cDNAof PpbZIP （GenBank accession No.： KC951877） was 1843 bp, containing an ORF of 1227 bp and encoding 408amino acids. PpbZIP also shared 92% homology with bZIP of Malus × domestica. QRT-PCR analysis showed thatthe expression of PpbZIP gene increased at initiate dormancy stage and then decreased with dormancy releasing.It was suggested that PpbZIP could involve in the regulation of bud dormancy process of ‘Huanghua’.Key Words： Pyrus pyrifolia ‘Huanghua’; bZIP; Clone; Expression
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2014年第2期172-178,共7页
Journal of Tropical and Subtropical Botany
基金
高等学校博士学科点专项科研基金项目(20113515110011)
福建省科技计划重点项目(2012N0005)资助
关键词
黄花梨
BZIP
克隆
表达
Pyrus pyrifolia ‘Huanghua'
bZIP
Clone
Expression
