黄皮果皮中的抗菌活性成分研究

Antibacterial Components from Peels of Clausena lansium(Lour.) Skeels

为了解黄皮[Clausena lansium(Lour.)Skeels]果皮中的抗菌活性成分,采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱等方法,从黄皮果皮的乙醇提取物中分离得到10个化合物,经波谱学分析鉴定为:lansine(1)、3-甲酰基咔唑(2)、3-甲酰基-6-甲氧基咔唑(3)、6-甲氧基咔唑-3-羧酸甲酯(4)、(6R,7E,9S)-9-羟基-4,7-巨豆二烯-3-酮(5)、7-羟基香豆素(6)、8-羟基呋喃香豆素(7)、辛黄皮酰胺(8)、对羟基肉桂酸甲酯(9)和胡萝卜苷(10)。其中化合物5和9为首次从黄皮属植物中分离得到,化合物8的碳谱数据是首次报道。用滤纸片琼脂扩散法测定化合物的抗菌活性,结果表明,化合物1、2、3、6、7和9对金黄色葡萄球菌(Staphylococcus aureus)有抑制作用。这有利于更好地开发利用黄皮这一药食两用的水果资源。

In order to understand the chemical constituents of Clausena lansium （Lour.） Skeels, ten compounds wereisolated from 95% EtOH extract of peels of C. lansium by silica gel and sephadex LH-20 column chromatography.On the basis of spectral data, their structures were identified as lansine （1）, 3-formyl carbazole （2）, 3-formyl-6-methoxy carbazole （3）, 6-methoxycarbazole-3-carboxylate （4）, （6R,7E,9S）-9-hydroxy-4,7-megastigmadien-3-one （5）, umbelliferone （6）, 8-hydroxyfurocoumarin （7）, zeta-clausenamide （8）, methyl p-hydroxycinnamate（9）, and daucosterol （10）. Compounds 5 and 9 were isolated from the genus Clausena for the first time, and the13C NMR spectral data of compound 8 was also reported for the first time. Compounds 1, 2, 3, 6, 7 and 9 showedantibacterial activity on Staphylococcus aureus in a bioactive assay by using paper disk diffusion method. Thesewould be helpful for exploitation of Clausena lansium.