PTEN和Syk在人大肠癌组织中的表达

Expressions of PTEN and Syk in human colorectal cancer tissues

目的:检测和分析PTEN和Syk蛋白在人大肠癌组织中的表达情况,探讨二者与大肠癌的发生、发展、浸润和转移间的关系。方法:采用免疫组织化学SP法检测60例人大肠癌组织及30例人正常大肠组织中PTEN和Syk的表达,分析二者与大肠癌患者临床病理特征之间的关系。结果:PTEN和Syk在人正常大肠组织中的表达阳性率分别为90%和96.67%,而在人大肠癌组织中的阳性率为36.67%和31.67%,差异有统计学意义(P<0.01)。两种蛋白的表达率均与患者的年龄、性别、肿瘤大小和发生部位无关(P>0.01),但与大肠癌的浸润程度、淋巴结转移、肿瘤分化程度及临床分期负相关(P<0.01)。人大肠癌中PTEN和Syk的阳性表达呈正相关(r=0.634,P<0.01)。结论:PTEN和Syk在人大肠癌组织中的表达与其发生、发展及浸润转移有密切关系,联合检测可作为判断人结直肠癌生物学行为的重要指标。

ObjectiveTo evaluate expression levels of PTEN and Syk in human colorectal cancer tissues and their relationshipswith the occurrence, development, metastasis, and prognosis of colorectal cancer. Methods：The expression levels of PTEN and Sykproteins in 60 cases with colorectal cancer and 30 health people were determined by the immunohistochemical SP method. Their corre-lations with clinic pathologic features were analyzed. Results： The positive rates of PTEN and Syk expression were 90% and 96. 67%in normal tissue, and 36. 67% and 31. 67% in the colorectal cancer tissues, respectively. The expression rates of the two types of pro-teins were significantly different between the two groups （P〈0. 01）. The expression levels of the two proteins in colorectal cancer tis-sues were not associated with patient age, gender, tumor size or tumor location （P〉0. 01）, but were significantly related with the dif-ferentiation degree of the cancer tissue, depth of invasion, lymph node metastasis, and Duke＇s staging（P〈0. 01）. The expression ofPTEN and Syk showed positive correlations in colorectal cancer（r=0. 634, P〈0. 01）. Conclusions： The expression levels of PTENand Syk in colorectal cancer are related to carcinogenesis, development, invasion, and metastasis. The joint detection of the two mark-ers can be used to determine the biological behavior and prognosis of colorectal cancer.揖