山奈酚分子印迹聚合物微球的制备及性能研究

Synthesis and evaluation of a molecularly imprinted polymer microsphere for kaempferol

为有效分离天然活性黄酮物质山奈酚,制备山奈酚分子印迹聚合物微球,以山奈酚为模板分子,4-乙烯基吡啶为功能单体,乙二醇二甲基丙烯酸酯为交联剂,乙腈为溶剂,采用扫描电镜、傅里叶红外光谱和热重分析进行表征,对其进行吸附性能测定。得到了最优的聚合反应条件是:反应温度60℃,功能单体与溶剂的体积比为1∶20,模板分子和功能单体的摩尔比为1∶8。合成的聚合物微球颗粒均匀,耐热性能好,对山奈酚具有良好的吸附能力,实际最大饱和吸附量是1.053mg/g。Scatchard分析表明聚合物对山奈酚存在特异性吸附性能。

In order to effectively separate the active natural flavonoids kaempferol (KAE),a molecularly imprinted polymer (MIP) microsphere was prepared in acetonitrile using KAE,4-vinyl pyridine (4-VP) and ethylene glycol dimethacrylate (EDMA) as the template,functional monomer and cross-linker respectively.Afterwards,the synthesized MIP was analyzed by SEM,FTIR and TG.The parameters studied for kaempferol include adsorption kinetics and adsorption isotherm.The optimum synthesis conditions of the MIP microsphere were as follows:synthesis temperature was at 60 ℃,the ratio of functional monomer to solvent was 1:20,and the ratio of template to functional monomer was 1:8.The experimental results showed that the MIP exhibited a favorable affinity for kaempferol,and the saturated capacity could reach to 1.053 mg/g.The Scatchard analysis revealed that the specific adsorption of kaempferols existed in MIP.