一株罗汉松内生真菌及其活性代谢产物

Identification of an Endophytic Fungus of Podocarpus macrophyllus and Its Antifungal Metabolites

植物内生真菌能代谢产生多种生物活性物质,本研究从浙江建德千年古树罗汉松Podocarpus macrophyllus中分离获得1株具有抗菌活性的菌株ZJDH255。通过形态和ITS rDNA序列分析,鉴定为稻黑孢霉Nigrospora oryzae。抑菌活性测试表明,菌株ZJDH255发酵粗提物对植物病原真菌灰葡萄孢Botrytis cinerea和瓜类炭疽菌Colletotrichum orbiculare具有较强抑制活性。采用正相硅胶和葡聚糖凝胶Sephadex LH-20进行柱层析从发酵粗提物中分离获得3个具抗真菌活性的化合物,通过质谱和核磁共振波谱技术将其结构鉴定为蜂蜜曲菌素mellein、灰黄霉素griseofulvin和去氯灰黄霉素dechlorogriseofulvin。

The endophytic fungal strain ZJDH255 with antifungal activity was isolated from Podocarpus macrophyllus in Jiande of Zhejiang, and was identified as Nigrospora oryzae based on its morphological characters and ITS rDNA sequence. Bioassay showed that the crude extract of the ZJDH255 fermentation broth exhibited strong inhibitory activities against plant pathogenic fungi Botrytis cinerea and Colletotrichum orbiculare. Three antifungal compounds were separated from the crude extract through normal-phase silica gel column chromatography and Sephadex LH-20 column chromatography. They were identified as mellein, griseofulvin and dechlorogriseofulvin based on mass spectrometry and nuclear magnetic resonance spectroscopy.