摘要
研究了甘氨酸预处理、菌龄、酶浓度、酶解温度及酶解时间对产琥珀酸放线杆菌(Actinobacillus succinogenes)ATCC 55618原生质体制备和再生的影响。确定出最佳条件为:在添加1.0%甘氨酸的培养基中培养菌体6 h,用浓度为0.08 mg/mL的溶菌酶于37℃酶解40 min。得到的原生质体形成率为96.28%,再生率为42.11%。目前,还没有关于产琥珀酸放线杆菌(A.succinogenes)ATCC 55618原生质体制备的相关研究,研究结果为后续的原生质体诱变及基因组改组技术奠定了基础。
The effect of glycine pretreatment, cell age and the concentration, temperature and work time of lysozyme on protoplast formation and regeneration by Actinobacillus succinogenes ATCC 55618 were studied. And the optimal conditions obtained as follow： the culture was cultivated 6 h in broth added with 1.0% glycine, treated by 0.08 mg/mL lysozyme for 40 min at 37 %：. As a result, the protoplast formation and regeneration were 96.28% and 42.11% respectively. The study of protoplast formation and regeneration by A. succinogenes ATCC 55618 has not been reported yet. This study played a fundamental role in protoplast technology and genome shuffling.
出处
《食品科技》
CAS
北大核心
2014年第6期30-34,共5页
Food Science and Technology
基金
吉林省科技发展计划项目(20126036)
关键词
产琥珀酸放线杆菌
原生质体制备
再生
Actinobacillus succinogenes
protoplast preparation
regeneration
