灰兜巴多糖的提取分离纯化研究

Extraction, isolation and purification of Huidouba polysaccharides

目的:对灰兜巴多糖进行提取、分离及纯化研究,为其做进一步的结构分析和对糖尿病的治疗提供新的线索。方法:采用水提醇沉法提取灰兜巴多糖;同时用Sevage法、木瓜蛋白酶-Sevage法和三氯乙酸法对其进行脱蛋白效果比较;最后用DEAE-52纤维素离子交换层析分离得到组分。结果:分别用苯酚-硫酸法和考马斯亮蓝G-250法测得灰兜巴粗多糖中的多糖含量为28.02%和蛋白质含量为1.23%;木瓜蛋白酶-Sevage法的蛋白质脱除率最高为96.08%;通过DEAE-52纤维素离子交换层析得到4个组分,总的多糖回收率为86.22%。结论:综合考虑选择木瓜蛋白酶-Sevage法为灰兜巴粗多糖脱蛋白的最佳工艺,HDB-2的多糖回收率较其他3个组分的要高,表明其溶解性较好。

Objective： Study on the extraction, isolation and purification of Huidouba polysaccharides, to provide new clues for further structural analysis and treatment of diabetes. Method： We used the water extraction and alcohol precipitation method to extract polysaccharides from Huidouba; Compared the effect of Sevage method, Papain-Sevage method and Trichloroacetic acid method on deproteinization. Huidouba polysaccharides were isolated by DEAE-Cellulose 52. Result： Respectively used phenol-sulfuric acid method and Coomassie brilliant blue G-250, The content of polysaccharide and protein is 28.02% and 1.23% in Huidouba polysaccharides. Protein removal rate of Papain-Sevage method up to 96.08%. We obtained four components through DEAE-Cellulose 52, the total polysaccharides recovery of 86.22%. Conclusion： Papain-Sevage method is the best method for Huidouba polysaccharides on deproteinization, polysaccharide recovery of HDB-2 is more high than other three components.