1株产C50类胡萝卜素极端嗜盐古菌的筛选鉴定及特性分析

Isolation,Identification and Characterization of Extremely Halophilic C_(50) Carotenoid-Producing Archaeon

从日晒盐场结晶池中筛选到1株产C50类胡萝卜素的红色极端嗜盐古菌。该菌株为革兰氏阴性菌，短棒状，最适生长盐度为250，最适生长pH为7。袁型鉴定方法结合16SrDNA序列分析判定，该菌属于极端嗜盐古菌盐红菌属Halorubrum，命名为Halorubrum sp．Sp1（16S rRNA Genbank登录号为KF697239）。根据紫外－可见光扫描特征光谱，确定该菌株主要色素为C50类胡萝卜素。pH8时单位细胞色素积累量最大，在盐度150－300范围内随盐度升高，单位细胞色素积累量逐渐降低。

An extremely halophilic C50 carotenoid-producing red archaeon was isolated from the crystalli-zer ponds in solar saltworks. The isolated strain is Gram-negative and short rod. The optimum salinityand pH for growth is 250 and 7, respectively. Phenotypic and molecular analyses of this strain indicatedthat it belonged to extremely halophilic archaea genus Halorubrurn and named Halorubrum Spl （16SrRNA Genbank registration number KF697239）. UV-visible scanning spectrum showed that C50 carotenoid was the major pigments presented in this strain. Pigment accumulation was maximizing at pH 8.In the salinity range of 150-300, increasing salinity resulted in declined pigment accumulation.