半胱氨酰天冬氨酸特异性蛋白酶-3在乙醇致小鼠睾丸损伤模型中的表达及意义

Significance and expression of Caspase-3 in ethanol-induced testis injury model

目的探讨半胱氨酰天冬氨酸特异性蛋白酶（Caspase）-3在乙醇致睾丸损伤中表达的变化，并探讨其意义。方法将24只雄性昆明小鼠随机分为对照组和实验组，每组12只，连续对实验组小鼠腹腔注射乙醇14d，建立睾丸损伤模型，处死所有小鼠并收集睾丸标本，应用实时定量聚合酶链反应（Real—time PCR）和Western blot检测Caspase-3 mRNA和蛋白的表达变化。结果Real-time PCR结果显示实验组Caspase-3 mRNA表达（3．35±0．13）显著高于对照组（P〈0．05）；Western blot结果显示Caspase-3蛋白表达（44．51±8．94）显著高于对照组（140．66±6．25，P〈0．05）。结论Caspase-3作为重要的促凋亡基因参与乙醇致睾丸损伤的过程。

Objective To explore the changes and significance of expression of Caspase-3 in the ethanol-induced testis injury model. Methods Twenty-four adult male mice were randomly divided into con- trol （n = 12） and ethanol （n = 12） groups. The mice in the ethanol group were given intra-peritoneal injection （IP） of ethanol at a dose of 3 g （ 15%, v/v） per kg body weight per day during the period of 14 days, to establish testicular damage model. All mice were sacrificed and the testes were collected. Real-time quantitative polymerase chain reaction （Real-time PCR） and Western blotting were applied to detect the expression of Caspase-3 mRNA and protein respectively. Results The results of Real-time quantitative PCR showed that the expression of Caspase-3 mRNA in ethanol group （3. 35 ±0. 13） was significantly higher than in control group （P 〈 0. 05）. Western blotting revealed that the Caspase-3 protein expression （44. 51 ± 8.94） was significantly higher in ethanol group than in control group （140. 66 ±6. 25, P 〈0. 05 ）. Conclusion Caspase-3 as an important pro-apoptotic gene may participate in the process of testicular damage by ethanol.