摘要
目的检测Ⅰ型胶原(CI)海绵/血管内皮生长因子(VEGF)缓释材料的组织相容性。方法将兔骨髓间充质干细胞(BMSCs)分别接种到Ⅰ型胶原(CI)、Ⅰ型胶原.血管生长因子(CI+VEGF)海绵材料及普通细胞培养板上,于第1~10天每天进行细胞计数试剂盒(CCK-8)法检测,于第5天进行扫描电镜观察。将1cm^2 CI海绵及CI+VEGF缓释海绵材料分别植入兔皮下,于第7、14、21、28天取材进行苏木素-伊红(HE)染色法分析。结果细胞计数试剂盒(CCK-8)分析显示在各个检测时间点,BMSCs增殖程度CI+VEGF组〉CI组〉单纯培养组,第4天3组细胞活力分别为2.77±0.21、2.21±0.17、1.86±0.13(P〈0.05);第8天分别为2.47±0.19、1.89±0.15、1.16±0.11(P〈0.05)。扫描电镜观察:有大量BMSCs黏附生长在CI+VEGF海绵支架表面及孔隙壁。HE结果显示CI+VEGF缓释海绵材料与CI具有相似的炎性反应,28d基本完全降解。结论CI+VEGF缓释海绵材料具有良好组织相容性。
Objective To investigate the histocompatibility of the type Ⅰ collagen ( CI)/vascular endothelial growth factor (VEGF) slow-release material. Methods Bone marrow-derived mesenchymal stem cells (BMSCs) were obtained with passage isolation, and then seeded on cross-linked CI, CI + VEGF materials and cell cuhure plate. Cytoeompatibility was detected by using scanning electron microscopy (SEM) at day 5 and cell counting kit-8 (CCK-8) test at every day during 10 days after seeding. The CI and CI + VEGF materials were subcutaneously implanted into 8 New Zealand albino rabbits. The rabbits were sacrificed at 7, 14, 21 and 28 days and the scaffolds and the surrounding tissues were extracted for hematoxylin-eosin (HE) staining. Results CCK-8 test revealed that the cell proliferation in 3 groups was as follows : CI + VEGF group 〉 CI group 〉 common culture group, and the cell viability in CI + VEGF group was significantly higher than that in controls ( day 4 : 2. 77 ± 0. 21 〉 2. 21 ± 0. 17 〉 1.86 ± 0. 13 P 〈 0. 05 ; day 8:2. 47 ± 0. 19 〉 1.89 ± 0. 15 〉 1.16 ± 0. 11, P 〈 0. 05 ). SEM image of the scaffolds showed that BMSCs spread well and homogenously distributed throughout the entire scaffolds. Histological FIE staining showed that the immune rejection of CI + VEGF sponge material was similar to CI, and CI + VEGF sponge material could be degradated at 28 days in animals. Conclusion CI + VEGF sponge materials have well histocomnatibilitv.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第6期1255-1257,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目(30973067)
广州市卫生局科研基金资助重点项目(201102A212003)
关键词
Ⅰ型胶原
骨髓间充质干细胞
血管内皮生长因子
组织相容性
Type Ⅰ collagen
Bone marrow-derived mesenchymal stem cells
Vascular endo-thelial growth factor
Histocompatibility