先天性脊柱裂子鼠羊水微小RNA谱差异性研究

Comparative microRNA profile analysis of amuiotic fluid in rats with congenital spina bifida

目的探讨先天性脊柱裂子鼠与正常发育子鼠羊水中的差异表达的微小RNA（miRNA）。方法利用全反式维甲酸构建先天性脊柱裂子鼠动物模型，采用miRNA芯片技术检测6例脊柱裂子鼠和6例正常发育子鼠羊水中的miRNA表达谱，筛选差异性表达miRNA，并采用实时定量聚合酶链反应（Real．timePCR）技术验证miRNA芯片结果的可靠性。结果相对于正常发育子鼠组，脊柱裂子鼠羊水中miRNA．9、miRNA-124a、miRNA-138表达显著下调（大于4倍），miRNA-134表达显著上调（大于4倍）。聚类分析显示，通过该4个差异表达显著的miRNA，可以将脊柱裂子鼠组和正常发育子鼠区分开。结论筛选得到的先天性脊柱裂相关miRNA可能在脊柱裂的发生过程中起重要调控作用。

Objective To explore the differentially expressed microRNA of amniotic fluid in rats with congenital spina bifida （CSB）. Methods Rats with CSB were induced by all-trans retinoic acid （ATRA）. microRNA （miRNA） microarray was applied to compare the miRNA expression profile between rats with CSB （ n = 6） and normal controls （ n = 6 ）. Real-time quantitative polymerase chain reaction （Real-time PCR）was used to confirm the results of microarray. Results As compared with normal controls, the expression of three microRNAs including miRNA-9, miRNA-124a and miRNA-138 was significantly decreased （more than 4 times）, while that of miRNA-134 was significantly increased in rats with CSB （more than 4 times）. Cluster analysis showed that the expression of these four miRNAs could distinguish the CSB group and the normal control group. Conclusion The differential expression miRNAs in rats with CSB maybe play an important role in the development of CSB.