促血管生成素-2在人大肠癌组织的表达及其临床意义

Expression of angiopoietin-2 in human colon carcinomas tissues and its clinical significance

目的观察促血管生成素-2（Ang-2）在大肠癌组织中的表达及其与临床病理的联系，并探讨其临床意义。方法采用逆转录-聚合酶链反应（RT—PCR）法检测40例大肠癌组织和正常肠黏膜中Ang-2 mRNA的表达，另用Western blot法检测其中10例肿瘤组织和正常组织中Ang-2蛋白的表达；用免疫组织化学法检测其中10例肿瘤组织中Ang-2蛋白的表达。结果60．0％（24／40）的大肠癌组织中Ang-2mRNA强表达，而正常肠黏膜中7．5％（3／40）弱表达，36例阳性表达的肿瘤组织中Ang-2 mRNA表达的平均吸光度值为4．3296±0．2809，而30例正常黏膜上皮的平均吸光度值为3．3097±0．2914，两者差异有统计学意义（P〈0．01）；Ang-2蛋白在大肠癌中的表达与肿瘤的病理类型、侵犯层次、淋巴结转移和病理分期有关，且随病程进展表达呈增强趋势。结论Ang-2参与了大肠癌组织新生血管的形成及维持，与大肠癌的进程一致。

Objective To evaluate the expression of angiopoietin-2 （Ang-2） in human colon carcinomas tissues and its relation with clinicopathological parameters in order to clarify the role of Ang-2 in the progression of tumors. Methods Forty cases of colon carcinomas were studied. The relationship between the mRNA expression of Ang-2 and clinicopathological features were examined by reverse transcrip-tion-polymerase chain reaction （RT-PCR） and Western blotting. Immunohistochemical method was used to detect the expression of Ang-2 protein in 10 samples of cancer tissue. Results Ang-2 mRNA expression was significantly detected in 60. 0% of human colon carcinomas tissues and 7.5% of normal tissues, the expression of Ang-2 mRNA in 36 cases of positive expression in tumor tissue, the average absorbance value was 4. 329 6± 0. 280 9, while the average absorbance of 30 normal mucosal epithelia/ values for 3. 309 7 ± 0. 291 4 （P 〈0. 01 ）. The Ang-2 expression was related to the pathological types, nodal invasion and lymph node metastasis stage of colon carcinomas. The patients with colon carcinoma strongly positive for Ang-2-expression showed more advanced stages or disease than those weakly positive for Ang-2 expression. Conclusion Ang-2 might be an important factor in tumor angiogenesis and growth in human colon carcinomas.