养心安神丸定性定量方法的研究

Study on the quantitative and qualitative methods of Yangxin Anshen pills

目的： 建立养心安神丸的定性定量方法。 方法： 采用TLC法鉴别丹参、首乌藤和南五味子；采用HPLC法测定南五味子中五味子酯甲的含量，使用C18（250 mm×4.6 mm，5 μm）色谱柱，以四氢呋喃-水（40：60）为流动相，流速1.0 mL·min^-1，检测波长250 nm。 结果： 在TLC色谱中可检出丹参、首乌藤和南五味子的特征斑点；五味子酯甲在0.082-0.82 μg范围内线性关系良好（r=0.9999），精密度试验RSD为0.56%（n=6），重复性试验RSD为1.4%（n=6），平均加样回收率为99.6%（RSD＝1.5%，n=6）。 结论： 实验结果表明本文建立的定性定量方法，可为养心安神丸的质量标准的制定提供参考。

Objective： To establish the quantitative and qualitative methods of Yangxin Anshen pills.Methods： TLC was used to identify Salviae Miltiorrhizae Radix et Rhizoma,Polygoni Multiflori Caulis and Schisandrae Sphenantherae Fructus.HPLC was used to determine the content of schisantherin A by C18 column（250 mm×4.6 mm,5 μm）,the mobile phase was tetrahydrofuran-water（40：60）at the flow rate of 1.0 mL·min^-1.The detection wavelength was set at 250 nm.Results： The characteristic spots of Salviae Miltiorrhizae Radix et Rhizoma,Polygoni Multiflori Caulis and Schisandrae Sphenantherae Fructus could be easily examined by TLC.The calibration curve of schisantherin A was linear in the range of 0.082-0.82 μg,r=0.9999.RSD of the precision test was 0.56%（n=6）,and RSD of the reproducibility test of this method was 1.4%（n=6）.The average recovery of schisantherin A was 99.6%（RSD=1.5%,n=6）.Conclusion： The experimental results show that the quantitative and qualitative methods established in this paper can be used to provide reference for the establishment of quality standard of Yangxin Anshen pills.