四川省6种竹叶部微生物的组成与结构分析

Microflora analysis on six kinds of bamboo leaves in Sichuan Province

采用传统林木病理学和现代分子生物学相结合的方法,对四川6种竹叶部微生物多样性和种类组成进行分析。结果表明:竹叶部微生物区系主要由真菌和细菌两大类群组成,且细菌数量高于真菌,但真菌的种类组成明显比细菌丰富;不同竹种间的微生物种群组成差异较大,雅安地区的真菌和细菌种类明显高于宜宾和泸州地区;由分离频率可以看出微生物各类群的分布呈现出季节性差异,在竹叶部持续存在的种群主要是枝孢霉菌Cladosporiumsp.、酵母菌Saccharomyces sp.、节菱孢菌Arthriniumsp.、格孢腔菌Pleosporales sp.、芽孢杆菌Bacillus sp.、假单胞杆菌Pseudomonas sp.。

Diversity and species composition of microorganism assemblages on the leaves of 6 spe cies of bamboo located in Sichuan Province were investigated based on the traditional forest pathology and modern molecular biology methods. The results show that fungi and bacteria are the large groups on the leaves. Although the bacterial quantity is larger than the amount of fungi, but the species of fungus are much more abundant than bacterial species. There are large differences among the six bamboo spe- cies about the microbial community structure. Yar an area is obviously higher than that of Yibin and Luzhou area. All kinds of groups present seasonal differences which can been seen from the separating frequency. Cladosporium sp. , Saccharomyces sp. ,Arthrinium sp. , Pleosporales sp. , Bacillus sp. and Pseudomonas sp. are the species existing persistently on the leaves. Finally, some biocontrol strains and beneficial microbes and even potential pathogenic microorganisms are obtained from bamboo leaves. In conclusion, this study provides the basis for the screening of bio-control strains, developing microorganism resource and preventing the diseases.