EphrinA1在输卵管妊娠组织中的表达

The expression of EphrinA1 in tubal ectopic pregnant tissues

目的研究EphrinA1在输卵管妊娠组织中的表达。方法 14例输卵管壶腹部妊娠组织分为种植部位组(n=14)和非种植部位组(n=14),对照组为分泌期输卵管壶腹部组织(n=12),应用免疫组化法和逆转录聚合酶链反应法检测3组组织中EphrinA1蛋白和mRNA的表达。结果 EphrinA1表达于输卵管上皮细胞及种植部位滋养细胞的胞浆,EphrinA1蛋白及mRNA在输卵管妊娠种植部位表达明显低于非种植部位和分泌期输卵管壶腹部对照组(P<0.05)。结论 EphrinA1在输卵管妊娠种植部位的低表达可能与输卵管妊娠发生有关。

This study designed to evaluate the expressions of EphrinA1 in implantation and non-implantation sites of tubal ectopic pregnant tissues. Samples of tubal ectopic pregnant tissues （n =14） were divided into implantation site group （n=14） and non-implantation site group （n=14）. Samples of oviductal ampullae tissues in the secretory stage as the control group （n=12） were obtained from women undergoing abdominal hysterectomy with adnexectomy for benign disease such as uterine leiomyoma, adenomyosis in the pelvic cavity. Expressions of EphrinA1 were determined by the immunohistochemical and RT-PCR methods in the three groups. As results, positive staining of EphrinA 1 was observed in the cytoplasm of the epithelial cells in oviducts and the trophoblast in the implantation site. The expression levels of EphrinA1 protein and mRNA in the implantation site group were lower than those of the non-implantation site group or the control group. In conclusion, EphrinA1 level in the implantation site of tubal pregnant tissues is relative low, which may involved in aetiology of tubal pregnancy.