PTPMeg2抑制NIH3T3/STAT3CA细胞模型STAT3转录活性的研究

PTPMeg2 inhibits STAT3 transcriptional activity in NIH3T3/STAT3CA cell model

目的探讨磷酸酶PTPMeg2对NIH3T3/STAT3CA细胞模型的增殖及STAT3转录活性的影响。方法构建STAT3异常突变的细胞模型NIH3T3/STAT3CA,MTT法和裸鼠异体移植瘤实验分别用于观察PTPMeg2对NIH3T3/STAT3CA细胞体外和体内增殖的影响,免疫共沉淀实验用于PTPMeg2与STAT3CA相互作用的研究,双荧光素酶报告基因法用于转录活性的研究。结果 PTPMeg2对NIH3T3/STAT3CA细胞在体外和体内都具有增殖抑制作用,与对照组比较均具有明显的统计学意义。PTPMeg2对NIH3T3/STAT3CA细胞的转录活性具有抑制作用,而PTPMeg2的突变体(PTPMeg2C515S)和ShPTPMeg2则无效。结论 PTPMeg2对NIH3T3/STAT3CA细胞具有增殖抑制作用,其作用机制可能与抑制STAT3的转录活性有关。

Aim To investigate the NIH3T3/STAT3CA cell proliferation ability and the STAT3 transcriptional activity affected by PTPMeg2 . Methods MTT assay and xenograft nude mice model were used to investigate the NIH3 T3/STAT3 CA cell proliferation inhibited by PTPMeg2 in vitro and in vivo. Co-immunoprecipitation assay was used to measure the interaction between PT-PMeg2 and STAT3CA. STAT3 transcriptional activity was measured by dualluciferase assay. Results The NIH3 T3/STAT3 CA cell proliferation ability was signifi-cantly inhibited by PTPMeg2 in vitro and in vivo com-pared with the control group （ P 〈0.05 ） . The tran-scriptional activity was increased by PTPMeg2 , but not the PTPMeg2 mutant （PTPMeg2C515S） and the ShPT-PMeg2 . Conclusion PTPMeg2 plays a role in inhibi-ting the proliferation ability of NIH3 T3/STAT3 CA cells through inhibiting the STAT3 transcriptional activity.