保妇康对慢性盆腔炎大鼠ICMI-1、TGF-β1mRNA及炎性细胞因子表达的影响

Effect of Baofukang on expression of ICMI-1,TGF-β1mRNA in uterus of chronic pelvic inflammation rats and inflammatory cytokines in blood serum of chronic pelvic inflammation rats

目的：研究保妇康对慢性盆腔炎大鼠子宫粘膜细胞间黏附分子1（ICMI-1）和转化生长因子β1（（TGF-β1）mRNA表达及血清炎性细胞因子含量的影响,以探讨其作用机制。方法：将雌性大鼠随机分为7组,除正常组和假手术组外,其他各组采用混合细菌加机械损伤法建立慢性盆腔炎大鼠模型。保妇康高、中、低剂量组分别以2.07,4.14,8.28g/kg ig给药,连续给药4周,采用半定量荧光免疫技术（RT-PCR）法测定大鼠子宫组织ICMI-1和TGF-β1mRNA的表达,用ELASA法测定血清TNF-α和IL-6的含量。结果：与正常组相比,模型组大鼠子宫黏膜炎性细胞浸润明显,子宫组织ICMI-1（2.03±2.76）和TGF-β1（9.03±2.76）mRNA的表达均明显升高（P〈0.05）。与模型组比较,保妇康2.07~8.28g/kg剂量范围内,均可明显减轻子宫粘膜炎症反应,高剂量组（8.28g/kg）、中剂量组（4.14g/kg）ICMI-1和TGF-β1mRNA的表达均明显降低,其中,高剂量组TGF-β1mRNA（4.11±1.29）的表达下降最明显;低剂量组（2.07g/kg）ICMI-1和TGF-β1mRNA的降低没有显著性差异。高、中剂量组（4.14~8.28g/kg）的IL-6和TNF-α明显降低。结论：保妇康能降低血清炎性细胞因子的含量,下调子宫组织ICMI-1和TGF-β1mRNA的表达,减轻盆腔组织粘连,这可能是其防治慢性盆腔炎的机制之一。

Objective： To study Effect of Baofukang on expression of ICMI-1 ,TGF-β1 mRNA in uterus and inflammatory cytokines in blood serum of chronic pelvic inflammation rats, and to explore possible mechanism of the therapeutic effects. Method： Female rats were randomly devided into 7 groups,in which chronic pelvic inflammation model was made by injection of a mixture of bacteriums suspension into injured uterus except the normal group and sham operation group. Baofukang（2.07, 4.14, 8.28g/kg/d ig）were administrated for 4 weeks. Expression of IC- MI-1 and TGF-β1 mRNA of ophoron and uterus in rats dectectod by RT-PCR. Histopathologic changes of uterus were observed by microscope. IL-6 and TNF-a were measured by enzyme-linked immunosorhent assay （ELISA）. Result：In compare with normal group, content of ICMI-1 （2.03 ± 2.76） and TGF-β1 （9.03 ± 2.76） mRNA in model group were rlsed obviously（ P 〈 0. 05 ）. after Baofukang administrated, Hemorheology of rats was improved obviously in all groups（ 2.07± 8.28g/kg）. the expression of ICMI-I and TGF-131 mRNA in high（8.28g/kg） and middle（4. 14g/kg） group were lowed severally（ P 〈 0.05 ）. however, there was no statistically significant difference between low （2.07g/kg） and model group（ P 〉 0.05 ）. Content of IL-6 and TNF-ctwere decreased obviously in high and middle groups （4.14 - 8.28g/kg）. Conclu- sion： The it can prevent and treat chronic pelvic inflammation by decreasing the expression of ICMI-1 and TGF-β1mRNA in ophoron and ute- rus of chronic pelvic inflammation rats and relieving pelvic adhesions.