半夏泻心汤对糖尿病胃轻瘫大鼠胃动力调控机制的研究

Study of Banxiaxiexin Decoction on gastric motility regulation mechanism of the rat diabetic gastroparesis

目的：探讨半夏泻心汤对糖尿病胃轻瘫大鼠胃动力的调控机制。方法：采用STZ法建立糖尿病大鼠模型,并分为模型组、吗丁啉组（0.03g/kg）、二甲双胍组（1.80g/kg）、半夏泻心汤高（10.20g/kg）、中（5.10g/kg）、低（2.55g/kg）剂量组,另设正常动物为空白组,灌胃一周后测定各组大鼠体重变化、胃肠排空率和肠推进率,胃窦组织c-kit表达情况以及胃动素、sp物质含量。结果：半夏泻心汤浓度在2.55~10.20g/kg对糖尿病胃轻瘫大鼠胃动力具有调控作用,与模型组相比,吗丁啉组、二甲双胍组、半夏泻心汤高剂量组、半夏泻心汤中剂量组给药后鼠重增加,半夏泻心汤高剂量组大鼠胃排空率降低;二甲双胍组、半夏泻心汤中剂量组肠推进率降低;二甲双胍组、半夏泻心汤高剂量组、半夏泻心汤中剂量组大鼠胃窦组织c-kit表达增加;二甲双胍组、半夏泻心汤高剂量组、半夏泻心汤中剂量组、半夏泻心汤低剂量组大鼠胃窦组织MOT、SP含量增加。结论：半夏泻心汤对糖尿病大鼠胃动力的调控作用可能与促进c-kit表达和MOT、SP的分泌有关。

Objective： To explore Banxiaxin decoction on gastric motility regulation mechanism of the rat diabetic gastroparesis. Methods： The rats dealed by STZ method were rannomly divided into model group,domperidone treatment group（ 0. 03g /kg）,metformin treatment group（ 1. 80g /kg）,Banxiaxiexin decoction treament group dose of high（ 10. 20g /kg）,Banxiaxiexin decoction treament group dose of middle（ 5. 10g /kg）,Banxiaxiexin decoction treament group dose of low（ 2. 25g /kg）,besides of normal group. Such indexes as content of rat weight,stomach residue rate,express of gastro-c-kit,motilin（ MOT）,substance P（ SP） were tested. Result： Banxiaxiexin Decotion concentration of 2. 55 ~ 10.20g /kg gastricmotility in diabetic gastroparesis with a regulatory role. Compared with the modle group,Domperidone treatment group,the metformin treatment group,Banxiaxiexin decoction treament group dose of high,Banxiaxiexin decoction treament group dose of middle of rat weight increased（ P ＆lt; 0. 05）,metformin treatment group,Banxiaxiexin decoction treament group dose of high stomach residue ratedecreases（ P ＆lt; 0.05）,metformin treatment group,Banxiaxiexin decoction treament group dose of high,Banxiaxiexin decoction treament group dose of middle express of gastro-c-kit increased（ P ＆lt; 0. 05）,metformin treatment group,Banxiaxiexin decoction treament group dose of high,Banxiaxiexin decoction treament group dose of middle,Banxiaxiexin decoction treament group dose of low MOT and SP increased（ P ＆lt; 0. 05）. Conclusion：The secretion of Banxiaxiexin decoction on the regulation of gastric motility in diabetic tats may promote expression of c-kit and MOT、SP.