柯孜木克颗粒剂对急性逆行性肾盂肾炎大鼠的影响

Study of Kezimuke Granules on acute retrograde pyelonephritis in the rat model

目的:研究新疆哈萨克民族药柯孜木克颗粒剂对急性逆行性肾盂肾炎的影响及其作用机制。方法:采用一侧输尿管短时间阻塞、膀胱内注射大肠杆菌制作急性逆行性肾盂肾炎大鼠模型,测定大鼠尿B2-微球蛋白(B2-MG)、尿分泌型免疫球蛋白a(sIgA)、血清白介素-10(IL-10)含量,以及做尿、肾组织的细菌学检查。结果:治疗两周后,柯孜木克颗粒剂(1.42、0.71、0.36g/kg)不同剂治疗组大鼠尿B2-MG均比三金片对照组及模型组明显为低,柯孜木克颗粒剂(1.42、0.71、0.36g/kg)不同剂量治疗组血清IL-10、尿sIgA均比三金片对照组及模型组明显为高,肾组织和尿细菌培养结果阳性率明显减少。结论:柯孜木克颗粒剂具有体内抑菌作用和增强机体免疫功能的双重作用。

Objective： To study the effect of Ke Zimuke Granules（ 柯孜木克颗粒剂） on acute retrograde pyelonephritis and its mechanism.Methods： Using unilateral ureteral obstruction for a short time,Escherichia coli was injected into the bladder to create an acute retrograde pyelonephritis rat model. The levels of rat urine B2- microglobulin（ B2-MG）,immunoglobulin a（ sIgA） and serum interleukin-10（ IL-10）were determined and bacteriological examination of urine and kidney tissue were carried out. Result： After two weeks of treatment,the treatment groups with various doses of Kezimuke granules displayed lower levels of urinary B2-MG than the San Jin tablet control group and the model group,with significant differences（ P ≤ 0. 05）. Levels of serum IL-10 and urinary sIgA were higher than that of the Sanjin tablet control group and model group,with a significant difference of（ P≤0. 05）. The rate of positive culture results for renal tissues and urine was reduced significantly. Conclusion： Kezimuke granules have the dual functions of in vivo antibacterial activity and enhancement of the immune.