髓系来源抑制细胞在胃癌患者外周血和肿瘤组织中的数量及其临床意义

The number of myeloid-derived suppressor cells in the peripheral blood and tumor tissues in patients with gastric cancer and its clinical significance

目的观察髓系来源抑制细胞(MDSC)在胃癌患者外周血的数量和肿瘤组织、癌旁组织的表达,探讨MDSC的数量与胃癌患者临床病理特征间的关系。方法收集62例胃癌患者外周血和其中12例患者的新鲜肿瘤组织和癌旁组织以及20份健康志愿者外周血,流式细胞仪分析HLA-DR-CD33+CD11b+MDSC的数量,采用One-way ANOVA和Mann-Whitney U检验以及t检验分析胃癌患者外周血MDSC的数量与肿瘤的浸润深度、分化程度、患者TNM分期和淋巴结转移的关系。结果和健康志愿者相比,初诊胃癌患者外周血MDSC数量增加(P<0.01);MDSC在胃癌患者外周血的数量与肿瘤的浸润深度、分化程度、患者TNM分期和淋巴结转移显著相关(P<0.05);MDSC在同一病人胃癌组织中的表达明显高于癌旁组织;复发/转移组患者外周血MDSC的数量明显高于无复发/转移患者(P<0.05);胃癌患者外周血HLA-DR-CD33+CD11b+MDSC高表达负性协同刺激分子T细胞免疫球蛋白黏蛋白分子3(Tim-3)。结论 MDSC在胃癌患者外周血的数量与肿瘤恶性程度及复发转移密切相关。

Objective To investigate the number of myeloid-derived suppressor cells （MDSCs） in peripheral blood, tumor tissue and para-tumor normal tissues in patients with gastric cancer in an attempt to explore the relationship between MDSCs expression and clinicopathologic characteristics. Methods Peripheral blood was collected from 62 gastric cancer patients and 20 healthy volunteers （HC group）. Gastric cancer tissues and adjacent normal tissues were obtained from 12 of the 62 gastric cancer patients. HLA-DR-CD33 ＋ CD11b＋ MDSCs were analyzed by flow cytometry. Student＇s t-test, One-way ANOVA and Mann-Whitney U test were used to explore the correlation between MDSCs expression in peripheral blood and the depth of tumor invasion, degree of differentiation, TNM stage and lymph node metastasis. Results Compare with the HC group, the number of MDSCs in peripheral blood of newly-diagnosed gastric cancer patients was higher （ P 〈 0.01 ）. The number of MDSCs in peripheral blood of gastric cancer patients was significantly associated with the depth of invasion, degree of differentiation, TNM stage and lymph node metastasis （P 〈0.05）. The expression of MDSCs from gastric cancer tissues was obviously higher than that of the adjacent tissues in the same patient. The number of MDSCs in peripheral blood from recurrent/metastasis group was obviously higher than that from non-recurrent/metastasis group （ P 〈 0.05）. In addition, compare with the HC group, the expression of negative costimulatory molecule T-cell immunoglobulin- and mucin-domain- containing molecule 3 （Tim-3） on HLA-DR-CD33＋ CD11b ＋ MDSCs in peripheral blood was higher in patients with gastric cancer. Conclusion MDSCs expression in peripheral blood of gastric cancer patients was closely associated with tumor malignant degree and tumor recurrence/metastasis.