中药合剂对早期糖尿病肾病大鼠足细胞nephrin和podocin表达的影响

Effect of TCM Mixture on Expression of Nephrin and Podocin in Podocytes of Early Diabetic Nephropathy Rats

目的:观察中药合剂(TCM mixture)对糖尿病肾病(DKD)大鼠足细胞裂孔隔膜蛋白分子(nephrin和podocin)表达的影响,探讨其对DKD的防治作用。方法:高脂高糖喂养8周联合小剂量STZ(30 mg/kg)建立T2DM大鼠模型,将DM模型大鼠随机分为中药合剂治疗组(B)及T2DM模型对照组(A);另设正常对照组(NC)。各组于治疗前及干预后4、8、12周末检测大鼠血糖浓度及尿微量白蛋白;第12周末称大鼠体重(BW)、肾重(KW),计算肾肥大指数(KW/BW),观察血清尿素氮(BUN)、肌酐(Scr)、总胆固醇(TC)、三酰甘油(TG)变化;光镜观察肾脏病理改变;应用免疫组化技术观察足细胞相关蛋白Nephrin、Podocin肾组织分布与表达;采用Western blot技术测定肾皮质Nephrin、Podocin蛋白的表达。结果:应用中药合剂干预后与T2DM模型对照组相比,大鼠的尿微量白蛋白总量、肾肥大指数、血糖、尿素氮、血肌酐较T2DM模型组明显降低(P<0.05),体重增加(P<0.05),病理改变减轻,nephrin和podocin蛋白表达增加(P<0.05)。结论:中药合剂能提高nephrin和podocin的表达,促进足细胞损伤修复,减少尿微量白蛋白的排泄,减缓糖尿病肾病的进展。

Objective：To observe the effects of Traditional Chinese Medicine mixture（ TCM mixture） on the expression of nephrin and podocin in podocytes of diabetic kidney disease （ DKD） rats. Methods：Rats were fed a high glucose and high fat diet and given a low dose of STZ to develop rat model of type 2 diabetes. Then the successful induced models randomly Divided into 2groups：TCM mixture treatment group and model group. In addition,the normal rats served as a normal control group. All the rats were received daily gavage respectively for 8 weeks. The concentration of blood glucose and urinary microalbumin were monitored before treatment and on week 4,8and 12 after treatment. twelve weeks later,the body weight （BW）,kidney weight（KW）,KW/BW,blood urea nitrogen （BUN）,serum creatinine （Scr）,cholesterol（CH） and Triglyceride（TG） were detected by biochemical methods. The pathological changes of kidney were also detected with optic microscope. The expression of nephrin and podocin in podocyte were detected by im-munohistochemistry. The protein levels of nephrin and podocin in the kidney tissue were also determined by western blotting. Results：after TCM mixture intervention,the levels of urinary microalbumin,renal index,blood glucose concentration,BUN and Scr in were sig-nificantly lower and body weight was higher than those in STZ group（P〈0. 05）. Meanwhile alleviating the damage of kidney. The ex-pression of nephrin and podocin were improved （P〈0. 05）. Conclusion：The TCM mixture protect kidney against possible related to improving the expression of the nephrin and podocin.