温经通络散外用对奥沙利铂所致周围神经毒性大鼠坐骨神经传导的影响

Effects of Wenjing Tongluo San for external use on sciatic nerve conduction in a rat model of oxaliplatin induced peripheral nerve toxicity

目的:观察外用温经通络散对奥沙利铂所致周围神经毒性大鼠坐骨神经的作用。方法:30只wistar大鼠,随机分为正常组、模型组、中药组。模型组和中药组腹腔注射奥沙利铂3mg/kg,隔天1次,共16次。从造模d1起给予中药组温经通络散浸泡大鼠四肢及尾部;观察机械和温度刺激下大鼠行为学变化及坐骨神经纤维变化,测坐骨神经传导速度变化。结果:中药组和模型组出现机械过敏和超敏反应,冷刺激缩足次数增多;坐骨神经传导速度减慢,中药组较模型组显著好转(P<0.01);模型组坐骨神经纤维出现脱髓鞘,甚至空泡变性,中药组较模型组减轻。结论:温经通络散能够明显缓解周围神经毒疼痛,加快坐骨神经传导速度,有效降低奥沙利铂对坐骨神经的损伤。

Objective：To explore the effects of Wenjing Tongluo San on sciatic nerve in a rat model of oxaliplatin induced chronic peripheral nerve toxicity.Methods：Thirty adult Wistar female rats were randomly divid- ed into three groups：blank group,model group and traditional Chinese medicine （TCM）group.In model group and TCM group,oxaliplatin was injccted at the dosage of 3mg/kg i.p every other day,a total of 16 times.In TCM group,Wenjing Tongluo San was administrated locally,limbs and tails of the rats were soaked in TCM soup daily from dl to d32.Mechanical and cold themal nociceptive behavior tests were observed,sciatic nerve fibers changes,sciatic nerve conduction velocity and pathological changes of sciatic nerve fibers were evaluated.Results：In model group,mechanical allodynia,mechanical hyperalgesia and cold themal allodynia were observed, sciatic nerve conduction velocity decreased.In addition,sciatic nerve fibers demyelination,even vacuoles degeneration were observed.In TCM group,these pathological changes were significantly relived,and sciatic nerve conduction velocity increased （P〈0.01）,compared to model group.Conclusion：Wenjing Tongluo San can alleviate chemotherapy-induced peripheral neuropathy significantly,increase sciatic nerve conduction velocity, reduce the rat sciatic nerve injury induced by oxaliplatin effectively.