间断低氧预适应对大鼠肝切除缺血再灌注肝脏中EPO的影响

Internittent hypoxic preconditioning effects EPO in the remaining liver after major hepatectomy

目的 观察术前间歇性低氧预适应对大鼠70％肝切术后肝脏中促红细胞生成素（Erythropoietin,EPO）量的影响.方法 健康清洁级SD大鼠120只,简单随机分为3组：假手术组（Sham,S组）40只;单纯大部肝切除组40只（Major hepatectomy,MH组）,即在肝门阻断下切除肝脏的左叶和中叶,肝门阻断20 min;间断低氧预适应组40只（Intermittent hypoxia preconditioning,IHP组）,术前1周将大鼠置于氧气体积分数10％的低氧环境中,每天1次,每次60 min.1周后在肝门阻断下行肝切除术（同IR组）.各组分别于术后2、6、12、24、48、72、120、168 h进行取材检测,用全自动生化分析仪检测下腔静脉血清ALT、AST含量,电镜下观察残余肝细胞中线粒体及内质网等的变化,采用ELISA测定残肝组织中促红细胞生成素的量,并运用统计学的方法比较各组中的意义.结果 MH组、S组、IHP组3组实验组中术后大鼠残肝组织中EPO水平具有显著统计学差异（P＜0.05）,间断低氧预处理组残余肝脏中EPO含量明显高于单纯肝切除组.结论 间断性低氧预适应可以促进肝切除术后残余肝组织中EPO的表达.

Objective To observe the effects of intermittent hypoxic preconditioning on the expression of apoptosis-related EPO after 70％ hepatectomy combined with ischemia-reperfusion injury.Methods One hundred and twenty healthy SD rats of clean grade,simple random divided into 3 groups：Sham operation group of 40 rats ; Pure major hepatic resection group was 40 （Major hepatectomy,MH）,namely in the hepatic portal blocking liver resection of the left and middle lobes,hepatic portal blocking 20 min ; intermittent hypoxia preconditioning group 40 （Intermittent hypoxia preconditioning,IHP group）.Results MH group,S group,IHP group,EPO level in three experimental groups in postoperative residual liver tissue in rats with significant difference （P ＜ 0.05）,Intermittent hypoxia preconditioning group of residual liver EPO content was significantly higher than that of simple hepatic resection group 40.Conclusion Intermittent hypoxia preconditioning can promote the expression of residual liver after hepatectomy in EPO.