玉郎伞查尔酮对异丙肾上腺素致小鼠心室重构的保护作用

Protective Effects of 17-Methoxyl-7-hydroxy-benzene-furabcgakcone on Isoproterenolinduced Ventricular Remodeling in Mice

目的研究玉郎伞查尔酮对异丙肾上腺素(ISO)致小鼠心室重构的保护并探讨其机制。方法 50只小鼠随机分为空白对照组,模型组,卡托普利组,玉郎伞查尔酮低、高剂量组,每组10只。除空白对照组外其他组采用连续7 d皮下注射ISO 1 mg·kg-1诱导小鼠心室重构模型。卡托普利组灌胃给予卡托普利65 mg·kg-1,玉郎伞查尔酮低、高剂量组尾静脉注射玉郎伞查尔酮2.5,5.0 mg·kg-1,给药10 d。实验结束时计算小鼠心脏、双肾、肺质量指数,观察心肌病理形态学改变,比色法测定血清中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,心肌组织羟脯氨酸(Hyp)含量。苏木精-伊红(HE)和Masson’s染色,分析系统测量心肌细胞横截面积(CSA)、心肌间质胶原容积分数(CVF)和血管周围胶原面积(PVCA)的变化。结果与空白对照组比较,模型组小鼠心脏、双肾、肺质量指数明显升高,血清SOD活性下降,血清MDA含量升高。与模型组比较,玉郎伞查尔酮组的心脏、双肾和肺质量指数明显降低,MDA含量明显降低(P<0.01或P<0.05)、SOD活性明显升高(P<0.01)、心肌组织中Hyp含量明显降低(P<0.01)。心肌胶原含量半定量分析显示,CVF和PVCA显著降低(P<0.01)。结论玉郎伞查尔酮对ISO诱导的小鼠心室重构具有保护作用,其机制可能与清除氧自由基,提高机体的抗氧化能力有关。

Objective To investigate the isoproterenol-induced ventricular remodeling in mice. effects of 17-methoxyl-7-hydroxy-benzene-furabcgakcone （MHBFC） on Methods Isoproterenol （ISO） was given subcutaneously （1mg·kg^-1, twice per day for 7 d） to induce ventricular remodeling in mice. Mice were divided into normal control group, model group, captopril group, MHBFC low and high-dose groups. 65 mg·kg^-1 captopril was given by intragastric administration in captopril group, 2.5,5.0 mg·kg^-1 MHBFC were given by intravenous injection in MHBFC low and high-dose groups. At the end of the 7th day, the hearts of the mice were weighted, and myocardial hypertrophy index was expressed as heart weight/body weight, double kidneys weight/body weight and lung weight/body weight （HW/BW, KW/BW and LW/BW）. Colorimetric method was used to determine the content of hydroxyproline （Hyp） in heart, the activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in serum. The histological changes were observed by HE and Masson＇s staining, the changes of cross section area （CSA）, collagen volume fraction, （CVF） and perivascular circumferential collagen area （PVCA） were determined. Results Compared with the model group, MHBFC potently inhibited cardiomyocyte hypertrophy, decreased the HW/BW, KW/BW and LW/BW, improved cardiac pathology changed, increased the of activity SOD, decreased the content of MDA in serum and the content of Hyp in heart tissue （P〈0.01 or P〈0.05）, decreased the CVF and PVCA （P〈0.01）. Conclusion MHBFC possesses protective effects against ISO -induced ventricular remodeling in mice, which may be related to its actions in reducing the oxidative stress and improving the antioxidant activity of the body.