野生山药内生真菌对瑞昌山药促生作用研究

Effect of endophytic fungi from Dioscorea opposite Thunb.on growth promotion of Ruichang yam

为探讨内生真菌对宿主植物的促生效应,采用Salkowski比色法,从48株由野生山药组织分离得到的内生真菌中筛选出4株具有分泌吲哚乙酸(IAA)活性的菌株(编号为YS07、YS16、YS21、YS27)。将4株内生真菌与瑞昌山药无菌苗共培养,观察菌丝在山药幼苗根中的定殖情况,并测定该菌株对瑞昌山药幼苗生长的影响。结果显示,4株内生真菌均能在一定程度上提高瑞昌山药无菌苗的鲜重、新生根数、根冠比和叶绿素含量,不同菌株在分泌IAA的能力和对瑞昌山药的促生作用方面存在差异,4株内生真菌促生作用的强弱依次为YS27、YS21、YS07、YS16。

To explore the promoting effect of endophyte fungi on the host plant, 4 IAA-producing strains （strain numbers： YS07, YS16, YS21, YS27）were obtained from 48 strains endophytic fungi of Dioscorea opposite Thunb. by Salkowski colorimetric. Aseptic seedlings of Ruichang yam and endophytic fungi were cultured together, and the growth of the seedlings and the endophytic fungi colonization in roots were observed. The results showed that the seedlings of Ruichang yam had significant increases in the fresh weight of plants, number of roots, root-shoot ratio and amount of chlorophyll which cultured with endophytic fungi. There were differences in IAA-producing and promoting effect among the endophytic fungi, the order of promoting effect of 4 strains was YS27〉YS21〉YS07〉YS16.