金属硫蛋白抗辐射的实验研究

THE EXPERIMENTAL STUDY ON ANTI RADIATION EFFECT OF METALLOTHIONEIN

目的 : 研究口服金属硫蛋白 (metallothionein,MT)对机体抵御电离辐射能力的影响。方法 : 采用灌胃方式给予受试小鼠 MT,连续灌胃 30 d后分别接受 7Gy、8Gy60 Co-γ一次性辐照及每周一次 1 Gy小剂量连续 8次辐照。于照后不同时间取血测定各组小鼠白细胞和淋巴细胞 ,并记录 8Gy一次性辐照后小鼠存活时间。结果 : (1 ) 7Gy辐照的实验条件对于抗辐射保健食品功能的观察较为适宜。 7Gy辐照后淋巴细胞的下降趋势与白细胞基本一致 ,淋巴细胞可以作为评价抗辐射保健食品实验的一项观测指标。 (2 )灌胃 MT0 .6mg/kg bw和 3mg/kg bw30 d的小鼠经 7Gy一次辐照后 ,白细胞和淋巴细胞数量明显高于未服 MT的辐照对照组。 (3)经 8Gy照射后服用 MT的小鼠比辐照对照组小鼠平均存活时间提高了 72 % (P<0 .0 5)。 (4)每周一次 1 Gy小剂量60 Co-γ射线照射小鼠 ,7次 (累计剂量 7Gy)和 8次 (累计剂量 8Gy)照射之后 ,服用 MT的小鼠白细胞和淋巴细胞数量明显高于未服 MT的辐照对照组小鼠。结论 : 口服 MT能够延长一次性大剂量电离辐射小鼠的存活时间 。

Ojbective: To study the anti ionizing radiation ability of metallothionein (MT) by oral administration. Methods: [WT5BZ]MT were given to mice for 30 days by stomach feeding, and then the mice received 7 Gy or 8 Gy 60 Co γ radiation, or received 1 Gy 60 Co γ small dose radiation once a day. The experiment lasted for 8 weeks. The number of leucocytes and lymphocytes were counted at different time after radiation. [WT5FZ]Results: [WT5BZ](1)7 Gy radiation was the most appropriate condition used for the experiments. After 7 Gy 60 Co γ radiation, the decrease of lymphocytes paralleled with that of leucocytes. Thus lymphocytes could be used as an indicator for evaluating the anti radiation effect of functional food. (2)After 30 days feeding of MT (0.6 mg/(kg bw·d) or 3.0 mg/(kg bw·d), the leucocytes and lymphocytes were significantly higher than that of control. (3)The survival time of MT accepted mice after 8 Gy 60 Co γ radiation was longer 72% than that of control (P<0.05). (4)After 1 Gy 60 Co γ radiation once a week for 7 or 8 weeks, the leucocytes and lymphocytes were significantly higher than that of control. [WT5FZ]Conclusion: [WT5BZ]Oral administration of MT can elongate the survival time of mice which accepted large dose of radiation, and reduce the damage on immunity system induced by large or multiple small dose radiation.