通过序列分析筛选单纯疱疹病毒I型蛋白组中的PACS-1结合蛋白

Screening for PACS-1 binding protein in herpes simplex virus-1 proteome by sequence analysis

目的筛选单纯疱疹病毒I型蛋白组中的PACS1结合蛋白。方法根据筛选标准,利用ScanProsite和PSORT软件对蛋白质进行分析,并结合相关文献中的实验数据对分析结果进行校正。结果该方法可有效地筛选出PACS1结合蛋白。利用该方法,在单纯疱疹病毒I型蛋白组中,共筛选出可能与PACS1结合的蛋白质33种。结论建立了一种有效的筛选PACS1结合蛋白的方法。筛选出的单纯疱疹病毒I型的PACS1结合蛋白,将为研究PACS1在疱疹病毒发生及潜伏性感染中的作用提供指导。

Aim To screen for PACS 1 binding protein in HSV 1 proteome in order to direct experimental research. Methods HSV 1 proteins were analysed by ScanProsite and PSORT software according to 4 screening criteria, then the results were modified by experimental data from references. Results The screening methods are effective. Total 33 proteins from HSV 1 proteome are identified as possible PACS 1 binding proteins. Conclusion An effective method for screening PACS 1 binding protein are established. The identified possible PACS 1 binding proteins of HSV 1 will provide instructions for experimental research on the role of PACS 1 in biogenesis and latent infection of HSV.