人乳头瘤病毒分型联合液基细胞学检测在宫颈病变筛查中的意义

The combination of Liquid based cytology and human papilloma virus testing for screening cervical lesions

目的探讨人乳头瘤病毒(HPV)分型联合细胞学检测在子宫颈病变筛查中的意义。方法收集疑似宫颈病变患者408例,分别进行细胞学和HPV基因芯片分型检测,了解各亚型HPV感染率及其与细胞学结果的相关性,判断HPV感染与年龄的关系,进行统计学分析。结果在高危型感染中居于前3位的分别是HPV 16、58、52。单一高危型HPV感染率随宫颈病变级别升高呈增加趋势,且差异有统计学意义(r=0.879,P<0.05)。高危型HPV感染率在年龄分布上呈两端高的趋势(<25岁和>50岁),但各年龄组间差异无统计学意义。结论 HPV分型检测宜联合细胞学应用于宫颈病变的筛查及临床治疗和随访。

Objective To evaluate the Liquid based cytology and human papilloma virus test for screening cervical lesions.Methods 408 suspected patients with cervical lesion from our gynecological outpatient service were collected.The liquid-based cytology and human papilloma virus（HPV）test were used,and the correlation of subtypes of HPV infection with cytology and age were observed.Results The top three HR-HPV genotypes were 16,58,52. The high-risk HPV infection rate increased with lesion grade（r=0.879,P〈0.05）.Conclusions The combination of HPV with liquid-based cytology testing should be applied in the screening,treatment and follow-up of cervical lesions.