CoCl2对NaCl胁迫下大麦生长及幼苗生理指标的影响

Influences of CoCl_2 on the growth and seedling physiological indexes of Hordeum vulgare under NaCl stress

为了明确钴对NaCl胁迫的缓解作用,本研究就CoCl2对不同浓度NaCl胁迫下大麦种子的萌发、幼苗生长及内源激素与相关生理指标的影响进行了探讨。结果表明,随着NaCl胁迫程度的加重,大麦种子发芽势、发芽率显著降低,芽与根的生长受到显著抑制。幼苗脱落酸(ABA)与丙二醛(MDA)大量积累,抗氧化酶活性提高。加入0.1mmol/L CoCl2显著提高了NaCl胁迫下大麦种子的发芽势与发芽率,促进了芽与根的生长,抑制了幼苗ABA的积累,同时提高了抗氧化酶活性,降低了MDA的含量,且在较严重NaCl胁迫下作用更明显。CoCl2提高了NaCl胁迫下幼苗(IAA+GA3)/ABA的值,减缓了活性氧(ROS)的积累与膜脂过氧化进程,对种子萌发及幼苗生长起到了促进作用,在一定程度上提高了大麦幼苗对NaCl胁迫的适应能力。

To determine the beneficial effect of cobalt on the growth of Hordeum vulgate under NaCl stress, the effects of addition of COCl2 on seed germination, seedling growth, endogenous hormones and some physiological indexes of barley seedlings under NaCl stress were studied. With the aggravation of NaCl stress, the germination vigor, germination capacity, shoot length and root length decreased considerably, while the contents of abscisic acid （ABA） and malondialdehyde （MDA） increased significantly, and the activities of antioxidant enzymes were enhanced. Under NaCl stress, germination vigor, germination capacity, shoot length and root length and the activities of antioxidant enzymes were elevated by the treatment of 0. 1 mmol/L COCl2, compared with NaCl stress alone, and it dramatically inhibited the accumulation of MDA and ABA. This effect was more significant under the more serious NaCl stress. The results showed that the addition of CoCl2 could improve （IAA＋GA3）/ABA, enhance the ability to clear up reactive oxygen species, reduce lipid peroxidation under NaCl stress, and promote seed germination, seedling growth and adaptive capacity of barley.