利用体外瘤胃发酵法研究全株玉米青贮与花生蔓和羊草间的组合效应

A study on the associative effect of whole corn silage-peanut vine and Leymus chinensis by rumen fermentation in vitro

本研究旨在利用体外瘤胃发酵法研究全株玉米青贮与花生蔓及羊草间的组合效应。将全株玉米青贮与花生蔓以100∶0,90∶10,80∶20,70∶30,60∶40,50∶50,40∶60,30∶70,20∶80,10∶90,0∶100的比例进行组合,筛选出全株玉米青贮与花生蔓的最优组合后再与羊草以这11个比例进行组合。利用体外瘤胃发酵技术,测定24h产气量、微生物蛋白产量及瘤胃干物质降解率的单项组合效应指数和多项组合效应指数。结果表明,1)在全株玉米青贮与花生蔓的不同比例组合中,产气量和干物质降解率单项组合效应指数均以70∶30的组合最大,分别为0.69和0.72,极显著高于其他比例的组合(P<0.01),微生物蛋白产量则产生了显著的负组合效应;2)在全株玉米青贮与花生蔓的不同比例组合中,多项组合效应指数以比例为70∶30的组合最大;3)在全株玉米青贮花生蔓最优组合(70∶30)与羊草的不同比例组合中,产气量和干物质降解率单项组合效应指数均以80∶20的组合最大,分别为0.17和0.18,极显著高于其他比例的组合(P<0.01),微生物蛋白产量则产生了显著的负组合效应;4)在全株玉米青贮花生蔓最优组合(70∶30)与羊草的不同比例组合中,多项组合效应指数以比例为80∶20的组合最大。综上,在本试验设计条件下,全株玉米青贮与花生蔓比例为70∶30的组合、全株玉米青贮-花生蔓-羊草比例为56∶24∶20的组合具有最大组合效应。

The experiment investigated the associative effect of whole corn silage （MCS）- peanut vine （PV） and Leymus chinensis （LC）. First, WCS was mixed with PV in the proportions of 100 ： 0, 90 ： 10, 80 ： 20, 70 ： 30, 60 ： 40, 50 ： 50, 40 ： 60, 30 ： 70 20 ： 80, 10 ： 90 and 0 ： 100. After the best combination of WCS and PV （BWP） was determined, the BWP was grouped with LC under these 11 proportions. Rumen fermentation in vitro was used and single factors associative effect indexes including the associative effects of gas production （AEGP）, microbiological crude protein （AEMCP）, dry matter degradation rate （AEDMD） and multiple factors associative effect indexes were analysed over 24 h. 1） Under the WCS-PV combination of different proportions, both AEGP and AEDMD were the largest at a ratio of 70 ：30 and they were 0.69 and 0.72 respectively. They were very significantly （P〈0.01） higher than those at other proportions, but AEMCP was negative. 2） Under the WCS-PV combination of different proportions, the 70 ： 30 ratio of WCS to PV had the largest associative effects according to the multiple factors associative effect indexes, and consequently, the 70 ： 30 ratio of WCS to PV was the best combination of WCS and PV （BWP） ; 3） Under the BWP-LC combination of different proportions, both AEGP and AEDMD were the largest at the ratio of 80 ： 20 and they were 0.17 and 0.18 respectively, very significantly （P〈0.01） higher than those at other proportions, and the AEMCP was negative. 4） Under the BWP-LC combination of different proportions, the 80 ： 20 ratio of BWP to LC had the largest associative effects. In conclusion, the WCS-PV combination at the ratio of 70 ： 30 and WCS-PV-LC combination at the ratio of 56 ： 24 ： 20 were found to have the largest associative effects in this study.