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MyHC基因在C2C12成肌细胞分化过程中的时序表达 被引量：2

Temporal expression of MyHC gene during C2C12 myoblast differentiation
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摘要 以体外培养的C2C12成肌细胞为研究对象,然后利用荧光定量PCR检测肌球蛋白重链(myosin heavy chains,MyHC)的3种亚型MyHC1、MyHC2x和MyHC2b在成肌诱导分化中(0-8 d)中的时序表达规律.结果表明,MyHC1基因在诱导分化的0-4 d表达呈上升趋势,4-8 d又呈下降趋势,在第4 d和第6 d的表达水平显著高于其他天数(p<0.05).MyHC2x基因在诱导分化的0-6 d表达呈上升趋势,但在第8 d又开始下降.MyHC2b在诱导分化的0-8 d中表达一直呈上升趋势,第6 d和第8 d表达水平显著高于其他天数(p<0.05). In this experiment, C2C12 myoblasts were used as the research object, which were cultured in vitro with 2 % horse serum to induce differentiation 0-8 d, and then real-time PCR was carried out to detect the expression of three subtypes of MyHC （myosin heavy chains） including MyHC1, MyHC2x and MyHC2b. The results showed that MyHC1 gene expression increases during day 0 to 4 after differentiation, while decreases during day 4 to 8. Moreover, the expression level on the 4th and 6th days is significantly higher than that of other days （p〈0.05）. MyHC2x gene expression is on the rise when it was induced differentiation from 0 to 6 days, but began to decline on the 8th day. The expression of MyHC2b was increasing when it was induced from 0 to 8 d and the expression of MyHC2b on the 6th and 8th days were significantly higher than the others（p〈0.05）
作者 林亚秋 张明 邬杨楠 李瑞文 郑玉才
机构地区 西南民族大学生命科学与技术学院 成都市妇女儿童中心医院生殖与不孕研究所
出处 《西南民族大学学报（自然科学版）》 CAS 2014年第3期350-353,共4页 Journal of Southwest Minzu University(Natural Science Edition)
基金 国家自然科学基金(No.31201990)
关键词 肌球蛋白重链基因 C2C12成肌细胞 时序表达 MyHC gene C2C12 Myoblasts temporal expression
分类号 Q593.3 [生物学—生物化学]
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参考文献15

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共引文献56

同被引文献31

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引证文献2

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西南民族大学学报（自然科学版）
2014年 第3期

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